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This was cloned in frame with the CDS of GFP into the BGLII-SalI site in pEGFP-N1 from Clontech for reports of cellular trafficking of mutated forms of FKHR

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Transfection of sub-confluent cells with DNA plasmids was executed with Lipofectamine 2000 in Opti-MEM...
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Importantly, all the off-focus on interactions concerned regions within the SOD-one minigene mRNA that ended up accessible to on-goal ASOs and that were predicted to be one stranded (Fig. 3A and Fig. S4A)

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The magnitude of the big difference in binding affinity among antisense oligonucleotides focusing on...