Re higher in cases with VTC at investigation of all cases (275683 vs. 250679 G/l, p = 0.001, t-test) (Fig. 1F), but this association was seen at separate investigation of tumor types only in SCC (282675 vs. 243681 G/l; p = 0.007, t-test) but not in AC (p = 0.077, t-test). No association of VTC with LMVD was seen in all cases and AC and SCC separately. (p.0.05, t- test). The presence of STC was associated with a higher PBPC at investigation of all cases compared to patients without STC (279688 G/l vs. 246676 G/l; p = 0.001, t- test) (Fig. 1G). At investigation of tumor types separately, such an association was found only in SCC (282675 G/l vs. 243682 G/l, p = 0.007, ttest), but missed significance in AC (2746101 G/l vs. 247673 G/ l, p = 0.077, t-test). The presence of STC was associated with higher LMVD in all cases (1667 vs. 1265 microvessels/field; p,0.001, t-test) (Fig. 1H) and also in AC (1666 vs. 1265 microvessels/field, p,0.001, t-test) and SCC (1767 vs. 1366, p = 0.002, t-test) separately. No direct association of STC or VTC with LVI was seen (p.0.05, Chi square test)., but in a linear regression model with LVI as dependent variable and including PBPC, STC and VTC showed that PBPC (p = 0.035, coefficient of regression,0.001) and VTC (p = 0.018, coefficient of regression 0,175) were associated with LVI. In a second linear regression model using LMVD as dependent variable, including the same independent variables, again PBPC (p = 0.034, coefficient of regression 20.009) and STC (p,0.001, coefficient of regression 5.415) influenced LMVD.investigation of tumor types separately, no such influence was found in AC, but at Autophagy analysis of SCC (p = 0.037, Breslow test, Fig. 2B). STC were associated with shorter DFS in multivariate analysis of AC (p = 0.022, Cox regression, table 2, Fig. 2C). No influence of STC was seen on OS at investigation of all cases, as well at investigation of AC and SCC separately (p.0.05, Breslow test or Cox regression, respectively; table 2). No relevance of VTC on DFS was seen at investigation of all cases. At investigation of AC and SCC separately, VTC was associated with shorter DFS in univariate analysis in SCC (p = 0.025, Breslow test, median DFS 506682 vs. 7946139 days; Fig. 2D), but associated with longer DFS in multivariate analysis of AC (p = 0.008, Cox regression, median DFS 29286990 vs. 7006141 days; Figure 2E). Presence of VTC was also associated with significantly shorter OS in SCC (p = 0.049, Breslow test, Fig. 2F). PBPC was not associated with DFS or OS in uni-or multivariate analysis (p.0.05, uni- or multivariate Cox regression, respectively).Cell CultureLECs were seeded at 1610`5 per 30 mm well, and after 24 hours isolated platelets were added at 3610`7, 10`6 or 10`5 per well and cells were cultured for another 48 h. As shown in Figure 3A , LEC cell count increased with the inhibitor number of added isolated platelets, indicating that LEC proliferation is enhanced by co-culture with human platelets in a dose-dependent manner. As second experiment, we investigated if LEC proliferation is enhanced by human platelets in a time-dependent manner. For this purpose, platelets at 1610`7 per well were added to isolated LECs (1610`5 per 30 mm well) and cells were cultured for another 24, 48 and 72 hours. Fig. 3F shows that LEC proliferation is enhanced by co-culture with human platelets in a timedependent manner compared to LECs without platelet addition. As next step, we investigated if pro-lymphangiogenic factors are.Re higher in cases with VTC at investigation of all cases (275683 vs. 250679 G/l, p = 0.001, t-test) (Fig. 1F), but this association was seen at separate investigation of tumor types only in SCC (282675 vs. 243681 G/l; p = 0.007, t-test) but not in AC (p = 0.077, t-test). No association of VTC with LMVD was seen in all cases and AC and SCC separately. (p.0.05, t- test). The presence of STC was associated with a higher PBPC at investigation of all cases compared to patients without STC (279688 G/l vs. 246676 G/l; p = 0.001, t- test) (Fig. 1G). At investigation of tumor types separately, such an association was found only in SCC (282675 G/l vs. 243682 G/l, p = 0.007, ttest), but missed significance in AC (2746101 G/l vs. 247673 G/ l, p = 0.077, t-test). The presence of STC was associated with higher LMVD in all cases (1667 vs. 1265 microvessels/field; p,0.001, t-test) (Fig. 1H) and also in AC (1666 vs. 1265 microvessels/field, p,0.001, t-test) and SCC (1767 vs. 1366, p = 0.002, t-test) separately. No direct association of STC or VTC with LVI was seen (p.0.05, Chi square test)., but in a linear regression model with LVI as dependent variable and including PBPC, STC and VTC showed that PBPC (p = 0.035, coefficient of regression,0.001) and VTC (p = 0.018, coefficient of regression 0,175) were associated with LVI. In a second linear regression model using LMVD as dependent variable, including the same independent variables, again PBPC (p = 0.034, coefficient of regression 20.009) and STC (p,0.001, coefficient of regression 5.415) influenced LMVD.investigation of tumor types separately, no such influence was found in AC, but at analysis of SCC (p = 0.037, Breslow test, Fig. 2B). STC were associated with shorter DFS in multivariate analysis of AC (p = 0.022, Cox regression, table 2, Fig. 2C). No influence of STC was seen on OS at investigation of all cases, as well at investigation of AC and SCC separately (p.0.05, Breslow test or Cox regression, respectively; table 2). No relevance of VTC on DFS was seen at investigation of all cases. At investigation of AC and SCC separately, VTC was associated with shorter DFS in univariate analysis in SCC (p = 0.025, Breslow test, median DFS 506682 vs. 7946139 days; Fig. 2D), but associated with longer DFS in multivariate analysis of AC (p = 0.008, Cox regression, median DFS 29286990 vs. 7006141 days; Figure 2E). Presence of VTC was also associated with significantly shorter OS in SCC (p = 0.049, Breslow test, Fig. 2F). PBPC was not associated with DFS or OS in uni-or multivariate analysis (p.0.05, uni- or multivariate Cox regression, respectively).Cell CultureLECs were seeded at 1610`5 per 30 mm well, and after 24 hours isolated platelets were added at 3610`7, 10`6 or 10`5 per well and cells were cultured for another 48 h. As shown in Figure 3A , LEC cell count increased with the number of added isolated platelets, indicating that LEC proliferation is enhanced by co-culture with human platelets in a dose-dependent manner. As second experiment, we investigated if LEC proliferation is enhanced by human platelets in a time-dependent manner. For this purpose, platelets at 1610`7 per well were added to isolated LECs (1610`5 per 30 mm well) and cells were cultured for another 24, 48 and 72 hours. Fig. 3F shows that LEC proliferation is enhanced by co-culture with human platelets in a timedependent manner compared to LECs without platelet addition. As next step, we investigated if pro-lymphangiogenic factors are.
