Ntly affected by the Bcl-2 genotype. The Bcl-2 protein is widely expressed during the development of the nervous system, but is principally retained in specific regions of the brain, including the cerebellum [45]. Hochman et al. [46] found that Bcl-2-knockout mice displayed increased susceptibility to cellular oxidative processes and a loss of neurons in the cerebellum, which suggest that neuronal viability in the cerebellum may be influenced by Bcl-2. Kaufmann et al. [25] found that level of Bcl-2 expression2.5 Statistical AnalysisStatistical analyses were performed using the SPSS 18.0 program (SPSS 22948146 Inc., Chicago, IL). Student’s t-test and Chi-square test were applied to compare the continuous and categorical variables between the two groups (A-carriers, and G homozygotes), Benzocaine respectively. Smoothed modulated gray matter segments were analyzed with SPM8 utilizing the framework of General Linear Model (GLM). To investigate whether Bcl-2 SNP exhibiting age-related linear interaction to alter regional GMV between two genotypic groups, voxel-wised covariate interaction analysis was employed using Bcl-2 genotype as a condition and age as covariates, controlling sex and education level as nuisance variables. This analysis tested for any regional GMV showing genotype-by-age interactions. To avoid possible partial volume effects around the margin between GM and WM, all voxels with a GM probability value lower than 0.2 (range from 0 to 1) were eliminated. The statistical criteria of interaction analysis were deemed to be significant at threshold of uncorrected p-value ,0.001 as well as extended cluster size more than 50 contiguous voxels. We used the icbm2tal function from the GingerALE toolbox (The BrainMap Development Team; http://brainmap. org/ale/index.html) to transform MNI coordinates into Talairach coordinates, and to minimize coordinate transformation discrepancy between MNI and Talairach space. Anatomical structures of the coordinates representing significant clusters were identified on the basis of the Talairach and Tournoux atlas [34]. All regional GMV were extracted and summed up from the peak coordinates showing significant differences.ResultsOf the 330 participants, 102 were G homozygotes, 65 had the A/A genotype, and 163 had 
the A/G genotype. There were no significant differences between the demographic and neuropsychological characteristics of the Bcl-2 G homozygotes and the Aallele carriers (Table 1). For GM volume, the Bcl-2 genotype was significantly associated with age-related changes in several brain regions. The association of the Bcl-2 rs956572 polymorphism with age was a predictor of regional GM volumes in the right cerebellum [F(1,328) = 13.77; P,.0001], the right SC 1 biological activity lingual gyrusBcl-2 and Age-Related Gray Matter Volume ChangesFigure 1. Interaction of Bcl-2 genotype and age on regional gray matter volume. Interaction of Bcl-2 15755315 genotype and age on (A) right cerebellum, (B) right lingual gyrus (BA17), (C) left lingual gyrus (BA18), (D) right middle temporal gyrus (BA19), and (E) right parahippocampal gyrus (hippocampus). (F) Showing the interaction results of voxel-wised covariate analysis using Bcl-2 genotype as a condition and age as covariates, controlling sex and education level as nuisance variables (uncorrected p,0.001, cluster size larger than 50). Abbreviations: MTG, middle temporal gyrus; BA, Brodmann Area. doi:10.1371/journal.pone.0056663.gwas higher in the central nervous system of older rats, especially in the cerebe.Ntly affected by the Bcl-2 genotype. The Bcl-2 protein is widely expressed during the development of the nervous system, but is principally retained in specific regions of the brain, including the cerebellum [45]. Hochman et al. [46] found that Bcl-2-knockout mice displayed increased susceptibility to cellular oxidative processes and a loss of neurons in the cerebellum, which suggest that neuronal viability in the cerebellum may be influenced by Bcl-2. Kaufmann et al. [25] found that level of Bcl-2 expression2.5 Statistical AnalysisStatistical analyses were performed using the SPSS 18.0 program (SPSS 22948146 Inc., Chicago, IL). Student’s t-test and Chi-square test were applied to compare the continuous and categorical variables between the two groups (A-carriers, and G homozygotes), respectively. Smoothed modulated gray matter segments were analyzed with SPM8 utilizing the framework of General Linear Model (GLM). To investigate whether Bcl-2 SNP exhibiting age-related linear interaction to alter regional GMV between two genotypic groups, voxel-wised covariate interaction analysis was employed using Bcl-2 genotype as a condition and age as covariates, controlling sex and education level as nuisance variables. This analysis tested for any regional GMV showing genotype-by-age interactions. To avoid possible partial volume effects around the margin between GM and WM, all voxels with a GM probability value lower than 
0.2 (range from 0 to 1) were eliminated. The statistical criteria of interaction analysis were deemed to be significant at threshold of uncorrected p-value ,0.001 as well as extended cluster size more than 50 contiguous voxels. We used the icbm2tal function from the GingerALE toolbox (The BrainMap Development Team; http://brainmap. org/ale/index.html) to transform MNI coordinates into Talairach coordinates, and to minimize coordinate transformation discrepancy between MNI and Talairach space. Anatomical structures of the coordinates representing significant clusters were identified on the basis of the Talairach and Tournoux atlas [34]. All regional GMV were extracted and summed up from the peak coordinates showing significant differences.ResultsOf the 330 participants, 102 were G homozygotes, 65 had the A/A genotype, and 163 had the A/G genotype. There were no significant differences between the demographic and neuropsychological characteristics of the Bcl-2 G homozygotes and the Aallele carriers (Table 1). For GM volume, the Bcl-2 genotype was significantly associated with age-related changes in several brain regions. The association of the Bcl-2 rs956572 polymorphism with age was a predictor of regional GM volumes in the right cerebellum [F(1,328) = 13.77; P,.0001], the right lingual gyrusBcl-2 and Age-Related Gray Matter Volume ChangesFigure 1. Interaction of Bcl-2 genotype and age on regional gray matter volume. Interaction of Bcl-2 15755315 genotype and age on (A) right cerebellum, (B) right lingual gyrus (BA17), (C) left lingual gyrus (BA18), (D) right middle temporal gyrus (BA19), and (E) right parahippocampal gyrus (hippocampus). (F) Showing the interaction results of voxel-wised covariate analysis using Bcl-2 genotype as a condition and age as covariates, controlling sex and education level as nuisance variables (uncorrected p,0.001, cluster size larger than 50). Abbreviations: MTG, middle temporal gyrus; BA, Brodmann Area. doi:10.1371/journal.pone.0056663.gwas higher in the central nervous system of older rats, especially in the cerebe.
