The liver an up-regulation was observed only in the group receiving DON without an Title Loaded From File adsorbing agent. In summary, DON caused oxidative stress in the small intestine. This has previously been reported in Caco-2 cells, where DON caused a significantly increased production of malondialdehyde, a biomarker of lipid peroxidation [49]. The hepatic effects of in vivo exposure to 10 mg/kg DON in broiler chickens have previously been reported by Frankic et al. (2006). They observed no differences in liver content of malondialdehyde, glutathione peroxidase and total antioxidant status, which are all markers for lipid peroxidation [50]. These findings suggest a more directgenotoxic effect of DON, rather than via the oxidative pathway [51,52]. Due to the damage to the intestinal barrier, an increased passage of non-invasive commensal bacteria may occur [53]. Both in duodenum and jejunum a significant up-regulation of TLR4 was observed 16574785 during our study, which suggests inflammation, more specific due to the presence of Gram-negative bacteria [54]. In contrast, no effects on TLR2 were observed. TLR2 is more affected by the presence of Gram-positive bacteria [55]. In the last part of the small intestine, the ileum, inflammation was caused by the presence of DON in combination with the adsorbing agent. In addition, in this group all the genes coding for the tight junction complex were also up-regulated and the same trend was observed for the gene XOR, coding for oxidative stress. Along the entire length of the small intestine administration of the adsorbing agent resulted in longer villi. From our qRT-PCR results, we can conclude that it is not the adsorbing agent that causes damage as no significant differences in gene expression were seen in the group receiving control feed in combination with the adsorbing agent. The adsorbing agent is a mineral clay and seems to protect DON from degradation by the gastric fluids and intestinal enzymes in the proximal part. This may result in a higher concentration of the mycotoxin in the distal part of the small intestine when an adsorbing agent is used. Thus the binding or interaction of DON with the adsorbing agent results in a longer exposure time of the intestine to DON. From our in vivo study, we can conclude that DON acts in a very specific way on the intestinal barrier in broiler chickens. Increased intestinal barrier permeability after chronic exposure to DON may lead to intestinal inflammation. The mechanism of action of DON can be different depending on the investigated target organ. The investigated mycotoxin adsorbing agent does not cause direct damage or irritation. However, feeding this clay mineral in combination with DON, may result in higher concentrations of the mycotoxin in more distal parts of the small intestine, resulting in damage of the intestinal barrier there.AcknowledgmentsWe would like to thank Delphine Ameye, Christian Puttevils, Jelle Lambrecht and Anja Van den Bussche for their skillful technical assistance.Author ContributionsConceived and designed the experiments: AO. Performed the experiments: AO. Analyzed the data: AO RS. Contributed reagents/materials/analysis 23977191 tools: AO VH. Wrote the Title Loaded From File manuscript: AO. Revised the manuscript: SC KC RD. Approved the manuscript: PDB SC KC RD.
Inflammatory cells that constitute the cancer microenvironment can limit or stimulate tumor growth. In cancers that are responsive to immune targeting, cytotoxic T lymphocytes are the major effector cells mediating a.The liver an up-regulation was observed only in the group receiving DON without an adsorbing agent. In summary, DON caused oxidative stress in the small intestine. This has previously been reported in Caco-2 cells, where DON caused a significantly increased production of malondialdehyde, a biomarker of lipid peroxidation [49]. The hepatic effects of in vivo exposure to 10 mg/kg DON in broiler chickens have previously been reported by Frankic et al. (2006). They observed no differences in liver content of malondialdehyde, glutathione peroxidase and total antioxidant status, which are all markers for lipid peroxidation [50]. These findings suggest a more directgenotoxic effect of DON, rather than via the oxidative pathway [51,52]. Due to the damage to the intestinal barrier, an increased passage of non-invasive commensal bacteria may occur [53]. Both in duodenum and jejunum a significant up-regulation of TLR4 was observed 16574785 during our study, which suggests inflammation, more specific due to the presence of Gram-negative bacteria [54]. In contrast, no effects on TLR2 were observed. TLR2 is more affected by the presence of Gram-positive bacteria [55]. In the last part of the small intestine, the ileum, inflammation was caused by the presence of DON in combination with the adsorbing agent. In addition, in this group all the genes coding for the tight junction complex were also up-regulated and the same trend was observed for the gene XOR, coding for oxidative stress. Along the entire length of the small intestine administration of the adsorbing agent resulted in longer villi. From our qRT-PCR results, we can conclude that it is not the adsorbing agent that causes damage as no significant differences in gene expression were seen in the group receiving control feed in combination with the adsorbing agent. The adsorbing agent is a mineral clay and seems to protect DON from degradation by the gastric fluids and intestinal enzymes in the proximal part. This may result in a higher concentration of the mycotoxin in the distal part of the small intestine when an adsorbing agent is used. Thus the binding or interaction of DON with the adsorbing agent results in a longer exposure time of the intestine to DON. From our in vivo study, we can conclude that DON acts in a very specific way on the intestinal barrier in broiler chickens. Increased intestinal barrier permeability after chronic exposure to DON may lead to intestinal inflammation. The mechanism of action of DON can be different depending on the investigated target organ. The investigated mycotoxin adsorbing agent does not cause direct damage or irritation. However, feeding this clay mineral in combination with DON, may result in higher concentrations of the mycotoxin in more distal parts of the small intestine, resulting in damage of the intestinal barrier there.AcknowledgmentsWe would like to thank Delphine Ameye, Christian Puttevils, Jelle Lambrecht and Anja Van den Bussche for their skillful technical assistance.Author ContributionsConceived and designed the experiments: AO. Performed the experiments: AO. Analyzed the data: AO RS. Contributed reagents/materials/analysis 23977191 tools: AO VH. Wrote the manuscript: AO. Revised the manuscript: SC KC RD. Approved the manuscript: PDB SC KC RD.
Inflammatory cells that constitute the cancer microenvironment can limit or stimulate tumor growth. In cancers that are responsive to immune targeting, cytotoxic T lymphocytes are the major effector cells mediating a.
