Rom strained ECs on the staticVSMCs (Figure 1D). The results revealed that compared with the static control, Rab28 expression in ECs was significantly increased by the CM from VSMCs subjected to cyclic strain, and the increase in 15 strain group was significantly higher than 5 strain (Figure 1E). In contrast, Rab28 expression in VSMCs did not show any significant change after treated with the CM from ECs subjected to 0, 5, or 15 cyclic strain (data not shown).Angiotensin II type 1 Receptor (AT1R) Blockers Attenuate the CM-induced Rab28 ExpressionAngiotensin II (Ang II) is one of the most important vasoactive molecules secreted from vascular tissue in situ and plays key roles in vascular remodeling through paracrine effects during hypertension [24,25]. We hypothesized that Ang II might be the crucial molecule that participated in the modulation of Rab28 expression in ECs by the CM from VSMCs subjected to high cyclic strain. ELISA revealed that Ang II concentration in the CM from VSMCs increased in a strain-amplitude dependent manner. The physiological 5 cyclic strain caused an increase in Ang II secretion from VSMCs in comparison to the static control, and the pathological 15 cyclic strain caused a much greater increase in Ang II secretion (data not shown). The expression of Rab28 in ECs subjected to CM from VSMCs was attenuated by bothFigure 1. Cyclic strain 1531364 regulated Rab28 expression in vascular cells in vitro and the conditioned media (CM) from VSMCs induced Rab28 expression in ECs. (A) Schematic drawing of the cyclic strain Anlotinib loading system in vitro, FX-4000. Vascular cells (VSMCs or ECs) were seeded on the elastic membrane of the culture plate. A pump produced periodic vacuum beneath the membrane to create the cyclic strain with elongation of the vascular cells. (B) Compared with the static group (0 elongation), Rab28 expression in VSMCs was not significantly altered by 5 cyclic strain, but increased significantly by 15 cyclic strain. (C) In ECs, the expression of Rab28 was not directly changed by cyclic strain application. (D) Schematic drawing of the CM experiments. The CM from VSMCs under different cyclic strain applications was transferred to static cultured ECs. (E) The Rab28 expression in ECs was significantly increased in response to the CM from VSMCs subjected to 15 cyclic strain, compared with those from 5 and 0 cyclic strain. (F) The induced expression of Rab28 in ECs by CM was attenuated by AT1R blockers, 1026 mol/L Irbesartan and 1025 mol/L Eprosartan. All results are given as mean 6 s.d., *P,0.05, n = 7 each. CS, cyclic strain; CM, conditioned medium. doi:10.1371/journal.pone.0056076.gRab28 Involved in NF-kB Nuclear Transport1026 mol/L Irbesartan and 1025 mol/L Eprosartan, the specific AT1R blockers (Figure 1F). Rab28 expression in ECs was also induced by exogenous Ang II stimuli in a concentration-dependent manner (Figure S2).into the nucleus (Figure 4C). Since it has been shown that nuclear factor kappa B (NF-kB) has a similar translocation as Rab28 after Ang II stimuli, and NF-kB is a crucial regulator of EC (��)-Hexaconazole biological activity functions [26,27], the possible relationship between Rab28 and NF-kB was further examined.Role of Rab28 in EC and VSMC FunctionsTo evaluate whether the changed expression of Rab28 participated in the modulation of vascular cell functions, the expression of Rab28 was “knocked-down” by target siRNA transfection, and the migration, proliferation and apoptosis of ECs and VSMCs were then analyzed. The resul.Rom strained ECs on the staticVSMCs (Figure 1D). The results revealed that compared with the static control, Rab28 expression in ECs was significantly increased by the CM from VSMCs subjected to cyclic strain, and the increase in 15 strain group was significantly higher than 5 strain (Figure 1E). In contrast, Rab28 expression in VSMCs did not show any significant change after treated with the CM from ECs subjected to 0, 5, or 15 cyclic strain (data not shown).Angiotensin II type 1 Receptor (AT1R) Blockers Attenuate the CM-induced Rab28 ExpressionAngiotensin II (Ang II) is one of the most important vasoactive molecules secreted from vascular tissue in situ and plays key roles in vascular remodeling through paracrine effects during hypertension [24,25]. We hypothesized that Ang II might be the crucial molecule that participated in the modulation of Rab28 expression in ECs by the CM from VSMCs subjected to high cyclic strain. ELISA revealed that Ang II concentration in the CM from VSMCs increased in a strain-amplitude dependent manner. The physiological 5 cyclic strain caused an increase in Ang II secretion from VSMCs in comparison to the static control, and the pathological 15 cyclic strain caused a much greater increase in Ang II secretion (data not shown). The expression of Rab28 in ECs subjected to CM from VSMCs was attenuated by bothFigure 1. Cyclic strain 1531364 regulated Rab28 expression in vascular cells in vitro and the conditioned media (CM) from VSMCs induced Rab28 expression in ECs. (A) Schematic drawing of the cyclic strain loading system in vitro, FX-4000. Vascular cells (VSMCs or ECs) were seeded on the elastic membrane of the culture plate. A pump produced periodic vacuum beneath the membrane to create the cyclic strain with elongation of the vascular cells. (B) Compared with the static group (0 elongation), Rab28 expression in VSMCs was not significantly altered by 5 cyclic strain, but increased significantly by 15 cyclic strain. (C) In ECs, the expression of Rab28 was not directly changed by cyclic strain application. (D) Schematic drawing of the CM experiments. The CM from VSMCs under different cyclic strain applications was transferred to static cultured ECs. (E) The Rab28 expression in ECs was significantly increased in response to the CM from VSMCs subjected to 15 cyclic strain, compared with those from 5 and 0 cyclic strain. (F) The induced expression of Rab28 in ECs by CM was attenuated by AT1R blockers, 1026 mol/L Irbesartan and 1025 mol/L Eprosartan. All results are given as mean 6 s.d., *P,0.05, n = 7 each. CS, cyclic strain; CM, conditioned medium. doi:10.1371/journal.pone.0056076.gRab28 Involved in NF-kB Nuclear Transport1026 mol/L Irbesartan and 1025 mol/L Eprosartan, the specific AT1R blockers (Figure 1F). Rab28 expression in ECs was also induced by exogenous Ang II stimuli in a concentration-dependent manner (Figure S2).into the nucleus (Figure 4C). Since it has been shown that nuclear factor kappa B (NF-kB) has a similar translocation as Rab28 after Ang II stimuli, and NF-kB is a crucial regulator of EC functions [26,27], the possible relationship between Rab28 and NF-kB was further examined.Role of Rab28 in EC and VSMC FunctionsTo evaluate whether the changed expression of Rab28 participated in the modulation of vascular cell functions, the expression of Rab28 was “knocked-down” by target siRNA transfection, and the migration, proliferation and apoptosis of ECs and VSMCs were then analyzed. The resul.
