Es that affects the composition of the cell membrane is that of the epithelial-mesenchymal transition (EMT), which is where cells downregulate their cell-cell junctions and acquire spindle cell morphology [12,13]. The EMT plays an important role in development [14,15], particularly in gastrulation and neural crest migration [14]. A critical component is the loss of type I cadherins that maintain stable cell-cell contacts through adherens junctions and desmosomes [16,17]. To preserve cellular shape and polarity, the intracellular domains of cadherins connect to the actin cytoskeleton through a-catenin and b-catenin [18,19,20]. In most cases, this is associated with transcriptional repression of E-cadherin [21,22], which in turn increases cell invasiveness [13,22,23,24]. Several specific repressor factors have been identified, such as the zinc-finger domain-containing Snail and Slug factors [25], and the basic helix-loop-helix DMXAA biological activity factor Twist, all of which can bind to the so-called E-boxes within the cadherin-1 (CDH1) gene promoter [25,26]. Their function is regulated by oncogenic pathways, particularly by AKT, glycogen synthaseEMT and HER3 Predicts Elisidepsin Sensitivitykinase 3b (GSK-3b), NF-kB, RAS and SRC, some of which have been described as potential elisidepsin targets [15,27,28]. In this scenario, until the proposed above targets are validated, robust models that permit the identification novel predictive biomarkers are essential. To this end, and due to the increasing evidence supporting a role for the EMT in the progression of many cancer types, with critical roles in invasion and metastatic dissemination, we decided to 24272870 study both HER3 and EMT as new predictive markers of elisidepsin treatment sensitivity in a panel of breast and pancreatic cell lines. In this report, we show that continued exposure to elisidepsin is correlated with a downregulation of epithelial markers in four different cancer cell types (pancreatic, breast, lung and colon). This behavior is Hydroxydaunorubicin hydrochloride site further accompanied by several morphological and signaling changes, resulting in the upregulation of mesenchymal markers. Furthermore, we investigated the effect of the drug on the expression of HER proteins and systematically compared the elisidepsin sensitivity of cell lines overexpressing and knockingdown HER3 receptor. Finally, we identified HER3 expression as the most important sensitivity marker of elisidepsin studied.Results Cancer Cell Line Sensitivity to ElisidepsinWe performed cell viability assays in a panel of 12 cell lines (6 breast cancer cell lines and 6 pancreatic carcinoma cell lines) to determine if there was a correlation between epithelial or mesenchymal expression markers and cell sensitivity to elisidepsin. Cells were treated with increasing concentrations of the compound for 72 h. The half maximal (50 ) inhibitory concentration (IC50) values for elisidepsin, as measured by a crystal violet assay using a spectrophotometer, ranged from 0.075 to 14 mM within the cell line panel (Fig. 1A). According to the results of a previous paper from our lab and others [27,28], only those cells with an IC50 value under or equal to 1 mM are considered sensitive to the elisidepsin. MDA-MB-231, PANC-1 and MiaPaCa-2 cell lines were the only cell lines that had an IC50 value higher than 1 mM (6.5, 7.5 and 14 mM, respectively). The other cell lines were classed as being sensitive to the drug (with IC50 values ranging from 0.075 to 0.6 mM). The effect of elisidepsin is not c.Es that affects the composition of the cell membrane is that of the epithelial-mesenchymal transition (EMT), which is where cells downregulate their cell-cell junctions and acquire spindle cell morphology [12,13]. The EMT plays an important role in development [14,15], particularly in gastrulation and neural crest migration [14]. A critical component is the loss of type I cadherins that maintain stable cell-cell contacts through adherens junctions and desmosomes [16,17]. To preserve cellular shape and polarity, the intracellular domains of cadherins connect to the actin cytoskeleton through a-catenin and b-catenin [18,19,20]. In most cases, this is associated with transcriptional repression of E-cadherin [21,22], which in turn increases cell invasiveness [13,22,23,24]. Several specific repressor factors have been identified, such as the zinc-finger domain-containing Snail and Slug factors [25], and the basic helix-loop-helix factor Twist, all of which can bind to the so-called E-boxes within the cadherin-1 (CDH1) gene promoter [25,26]. Their function is regulated by oncogenic pathways, particularly by AKT, glycogen synthaseEMT and HER3 Predicts Elisidepsin Sensitivitykinase 3b (GSK-3b), NF-kB, RAS and SRC, some of which have been described as potential elisidepsin targets [15,27,28]. In this scenario, until the proposed above targets are validated, robust models that permit the identification novel predictive biomarkers are essential. To this end, and due to the increasing evidence supporting a role for the EMT in the progression of many cancer types, with critical roles in invasion and metastatic dissemination, we decided to 24272870 study both HER3 and EMT as new predictive markers of elisidepsin treatment sensitivity in a panel of breast and pancreatic cell lines. In this report, we show that continued exposure to elisidepsin is correlated with a downregulation of epithelial markers in four different cancer cell types (pancreatic, breast, lung and colon). This behavior is further accompanied by several morphological and signaling changes, resulting in the upregulation of mesenchymal markers. Furthermore, we investigated the effect of the drug on the expression of HER proteins and systematically compared the elisidepsin sensitivity of cell lines overexpressing and knockingdown HER3 receptor. Finally, we identified HER3 expression as the most important sensitivity marker of elisidepsin studied.Results Cancer Cell Line Sensitivity to ElisidepsinWe performed cell viability assays in a panel of 12 cell lines (6 breast cancer cell lines and 6 pancreatic carcinoma cell lines) to determine if there was a correlation between epithelial or mesenchymal expression markers and cell sensitivity to elisidepsin. Cells were treated with increasing concentrations of the compound for 72 h. The half maximal (50 ) inhibitory concentration (IC50) values for elisidepsin, as measured by a crystal violet assay using a spectrophotometer, ranged from 0.075 to 14 mM within the cell line panel (Fig. 1A). According to the results of a previous paper from our lab and others [27,28], only those cells with an IC50 value under or equal to 1 mM are considered sensitive to the elisidepsin. MDA-MB-231, PANC-1 and MiaPaCa-2 cell lines were the only cell lines that had an IC50 value higher than 1 mM (6.5, 7.5 and 14 mM, respectively). The other cell lines were classed as being sensitive to the drug (with IC50 values ranging from 0.075 to 0.6 mM). The effect of elisidepsin is not c.
