Ith the comparedntioxidants 2021, ten, x FOR PEER REVIEWFigure 3. Liver indices of indices
Ith the comparedntioxidants 2021, ten, x FOR PEER REVIEWFigure three. Liver indices of indices of channel catfish (Ictalurus punctatus) subject to various treatFigure three. Liver antioxidant antioxidant channel catfish (Ictalurus punctatus) subject to distinct therapy. (A) Malonment.(B) Superoxide dismutase, SOD; (C) Glutathione peroxidase, GPx; (D) Glutathione reductase, GR; dialdehyde, MDA; (A) Malondialdehyde, MDA; (B) Superoxide dismutase, SOD; (C) Glutathione peroxidase, GPx; (D) Glutathione reductase, GR; (E) Glutathione, GSH; (F) Total antioxidant capacity, T-AOC. (E) Glutathione, GSH; (F) Total antioxidant capacity, T-AOC. Data indicate the imply LY294002 PI3K Values of three replicate cages per Data indicate the imply values of 3 replicate cages per therapy (3 fish per replicate breedtreatment (3 fish per replicate breeding barrel). SignificanceANOVA (p 0.05) followed by Duncan’s multi- followed by ing barrel). Significance was evaluated by one-way was evaluated by one-way ANOVA (p 0.05) Duncan’s many range tests. Values marked with unique letters are significantly various between the treatple range tests. Values marked with distinct letters are drastically various in between the therapy groups. ment groups.three.6. Antioxidant-Related Gene Expression in the Liver As shown in Figure 4, there was no considerable difference in sod and gpx8 gene expression among the therapy groups. The OFO group remarkably downregulated gpx1, gr, and nrf2 mRNA expression, and upregulated keap1 mRNA expression (p 0.05) com-Antioxidants 2021, 10,9 of3.six. Antioxidant-Related Gene Expression inside the Liver As shown in Figure four, there was no important difference in sod and gpx8 gene expression amongst the therapy groups. The OFO group remarkably downregulated gpx1, gr, and nrf2 mRNA expression, and upregulated keap1 mRNA expression (p 0.05) compared with all the FFO group. gpx1, gr, and nrf2 mRNA expression in the FFOT group had been substantially Antioxidants 2021, ten, x FOR PEER Critique ten of 20 upregulated compared together with the OFO group, whereas the opposite result was observed for the mRNA expression degree of keap1 (p 0.05)Figure 4. Effects of dietary taurine on liver antioxidant-related genes expression of channel catfish (Ictalurus punctatus) fed Figure 4. Effects of dietary taurine on liver antioxidant-related genes expression of channel catfish (Ictalurus punctatus) fed oxidized-fish-oil diets. (A) sod; (B) gpx1; (C) gpx8; (D) gr; (E) keap1; (F) nrf2. Information indicate the imply values of three replicate oxidized-fish-oil diets. (A) sod; (B) gpx1; (C) gpx8; (D) gr; (E) keap1; (F) nrf2. Information indicate the imply values of three replicate cages per therapy (3 fish per replicate breeding barrel). Significance was evaluated by one-way ANOVA (p 0.05) cages per treatment (3 fish per replicate breeding barrel). Significance was evaluated by one-way ANOVA (p 0.05) followed by Duncan’s several range tests. Values marked with distinct letters are considerably distinctive amongst the followed by Duncan’s various variety tests. Values marked with unique letters are significantly unique involving the remedy groups. treatment groups.3.7. Histological Diversity Library web Structure within the Intestine 3.7. Histologicalin Figure 5, via the H E staining analysis in the intestine, the feeding As shown Structure inside the IntestineAs shown in Figure significantly H E staining analysis from the intestine, the feeding of of oxidized-fish-oil diets5, via the lowered the goblet.
