N these processes [5]. Against this background, we’ve CDK8 custom synthesis investigated the well-known
N these processes [5]. Against this background, we’ve got investigated the well-known effects of a diet enriched in n-3 PUFA as compared to a diet regime comprising mostly of saturated and monounsaturated fatty acids on glucose andPLOS A single | DOI:10.1371journal.pone.0114942 December 26,17 GPR120 Will not be Needed for n-3 PUFA Effects on Energy MetabolismTable three. Plasma analyses. Parameter\Genotype Total bilirubin (mM) Albumin (gl) ALAT (mkatl) Adiponectin (nM) Leptin (ngml) Cholesterol (mM) Triglycerides (mM) Fructosamine (mM) C20:5n-6 (mM) C22:6n-3 (mM) C14:0 (mM) C16:0 (mM) C16:1n-9 (mM) C18:0 (mM) C18:1n-9 (mM) C18:2n-6 (mM) C18:3n-3 (mM) C20:4n-6 (mM) n-3 of total WT (n58) SAT HFD 1.78.10 32.50.54 7.46.99 147.648.65 68.30.41 six.92.20 0.96.15 72.49.75 ,LLOQ 93.two.six 48.4.six 468.29.9 20.three.six 282.70.2 278.22.6 271.85.6 43.two.1 394.87.3 7.2.2 WT (n58) PUFA HFD 1.80.15 29.58.26 0.62.08 275.218.31 63.43.92 3.64.30 0.64.15 68.85.54 163.94.six 129.six.2 88.2.8 207.72.1 31.eight.9 119.1.five 67.13.7 22.9.1 88.7.1 91.6.9 38.2.eight Gpr120 KO (n57) SAT HFD 1.81.15 30.96.35 4.75.59 191.231.27 64.54.04 6.16.25 0.69.08 70.46.69 ,LLOQ 76.five.5 62.three.4 362.54.6 16.6.2 230.60.0 213.41.six 224.51.two 63.two.1 323.95.9 9.3.five Gpr120 KO (n57) PUFA HFD 1.81.12 29.56.61 0.50.04 335.680.46 52.36.17 two.90.16 0.55.08 67.30.37 137.3.4 110.9.5 114.eight.5 165.three.six 28.6.9 98.0.five 50.4.4 18.0.9 109.1.1 77.six.0 39.3.4 1-way ANOVA NS p,0.05 p,0.05 p,0.05 p,0.05 p,0.05 NS NS p,0.05 p,0.05 p,0.05 p,0.05 p,0.05 p,0.05 p,0.05 p,0.05 p,0.05 p,0.Values are presented as group mean SEM. Statistical evaluation performed by 1-way ANOVA followed by Students t-test comparing SAT HFD vs. PUFA HFD. Significance tests were produced amongst mice fed SAT HFD vs. mice fed PUFA HFD. p,0.05; p,0.01; p,0.001. Percentage of n-3 fatty acids in plasma triglycerides, phospholipids and cholesteryl esters were calculated as sum of 18:3n-3, 20:5n-3 and 22:6n-3 divided by the sum of all detected fatty acids. LLOQ – Reduce Limit Of Quantification. doi:ten.1371journal.pone.0114942.tenergy metabolism in a newly created Gpr120 deficient (Gpr120 KO) mouse line. We found that wild sort (WT) and Gpr120 KO mice created a comparable amount of obesity and impaired glucose control when fed a saturated HFD containing low amounts of n-3 PUFAs. To our surprise, when fed the n-3 PUFA enriched HFD, Gpr120 deficient mice displayed similar body weight, body fat mass, liver fat, energy metabolism and glucose control to WT mice on n-3 PUFA HFD, showing that GPR120 is dispensable for the optimistic effects of n-3 PUFA on metabolism. The effects of n-3 PUFA supplementation on body weight regulation and glucose manage in rodents are properly studied. In accordance with expectation, consumption of a high fat diet program (HFD) containing n-3 PUFA resulted in reduce physique weight gain and adipose tissue mass than feeding of a HFD containing mostly saturated fat in ALDH2 Source C57Bl6 mice [257]. Similarly, fat-1 transgenic mice that express a desaturase from C. Elegans, which converts n-6 PUFA to n-3 PUFA, also show reduced physique weight acquire [28] as in comparison to WT mice fed HFD. Our study showed that 11 weeks of PUFA HFD feeding resulted in reduced total physique fat mass in comparison with a corresponding group fed SAT HFD. Surprisingly, the weights ofPLOS 1 | DOI:10.1371journal.pone.0114942 December 26,18 GPR120 Is not Required for n-3 PUFA Effects on Energy Metabolismthe epididymal and retroperitoneal fat depots weren’t different in between the diets in the time of sacrifice, although the total body weight was.
