Name :
GRP78/BIP antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
GRP78/BIP Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IHC detected in human pancreas cancer tissue, human brain tissue, human breast cancer tissue, human colon cancer tissue, mouse heart tissue. Positive IF detected in HepG2 cells. Positive IP detected in MCF-7 cells. Positive WB detected in HepG2 cells, BxPC-3 cells, HeLa cells, MCF7 cells, rat liver tissue, TN treated Hela cells. Observed molecular weight by Western-blot: 78 kDa
Tested applications :
ELISA, WB, IP, IF, FC, IHC
Species reactivity :
Human, Mouse, Rat; other species not tested.
Alternative names :
BIP antibody; GRP 78 antibody; GRP78 antibody; HSP A5 antibody; HSPA5 antibody; MIF2 antibody
Immunogen :
Human GRP78,BIP Recombinant protein (GST tag)
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of GRP78/BIP recombinant protein (Accession Number: NM_005347). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:500-1:5000IHC: 1:20-1:200IF: 1:10-1:100
Background :
GRP78 (HSPA5), also referred to as ‘immunoglobulin heavy chain-binding protein’ (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.
References :
Gao XC, Zhou CJ, Zhou ZR. Co-chaperone HSJ1a dually regulates the proteasomal degradation of ataxin-3. PloS one. 6(5):e19763. 2011. Liu CI, Chen CC, Chen JC. Proteomic analysis of anti-tumor effects of 11-dehydrosinulariolide on CAL-27 cells. Marine drugs. 9(7):1254-72. 2011. Kim HJ, Kang UB, Lee H. Profiling of differentially expressed proteins in stage IV colorectal cancers with good and poor outcomes. Journal of proteomics. 75(10):2983-97. 2012. Hill R, Li Y, Tran LM. Cell intrinsic role of COX-2 in pancreatic cancer development. Molecular cancer therapeutics. 11(10):2127-37. 2012. Zhu Z, Xu W, Dai J. The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis. The international journal of biochemistry & cell biology. 45(3):571-82. 2013. Ho KY, Yeh TS, Huang HH. Upregulation of phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in acquired middle ear cholesteatoma growth. International journal of molecular sciences. 14(7):14439-59. 2013. Xue J, Wei J, Dong X. ABCG1 deficiency promotes endothelial apoptosis by endoplasmic reticulum stress-dependent pathway. The journal of physiological sciences : JPS. 63(6):435-44. 2013. Dong D, Zhang S, Yin L. Protective effects of the total saponins from Rosa laevigata Michx fruit against carbon tetrachloride-induced acute liver injury in mice. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 62:120-30. 2013.
Related websites: https://www.medchemexpress.com/antibodies.html
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