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Name :
ACAT1 antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
ACAT1 Rabbit Polyclonal antibody. Positive WB detected in human skeletal muscle tissue, human heart tissue. Positive IP detected in mouse heart tissue. Positive IF detected in Hela cells. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 45kd

Tested applications :
ELISA, WB, IHC, IF, IP

Species reactivity :
Human,Mouse,Rat; other species not tested.

Alternative names :
ACAT antibody; ACAT1 antibody; Acetoacetyl CoA thiolase antibody; MAT antibody; THIL antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of ACAT1 recombinant protein (Accession Number: BC010942). Purification method: Antigen affinity purified.

Clonality :
Polyclonal

Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:200-1:2000IHC: 1:20-1:200IF: 1:10-1:100

Background :
ACAT1 represents acetyl-Coenzyme A acetyltransferase 1

References :
Yao J, Chen S, Mao Z, Cadenas E, Brinton RD. 2-Deoxy-D-glucose treatment induces ketogenesis, sustains mitochondrial function, and reduces pathology in female mouse model of Alzheimer’s disease. PloS one. 6(7):e21788. 2011. Peralta S, Torraco A, Wenz T, Garcia S, Diaz F, Moraes CT. Partial complex I deficiency due to the CNS conditional ablation of Ndufa5 results in a mild chronic encephalopathy but no increase in oxidative damage. Human molecular genetics. 23(6):1399-412. 2014. Hao S, Ji J, Zhao H. Mitochondrion-Targeted Peptide SS-31 Inhibited Oxidized Low-Density Lipoproteins-Induced Foam Cell Formation through both ROS Scavenging and Inhibition of Cholesterol Influx in RAW264.7 Cells. Molecules (Basel, Switzerland). 20(12):21287-97. 2015.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
Related websites: https://www.medchemexpress.com/antibodies.html
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Author: Gardos- Channel