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Name :
HDAC3 antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
HDAC3 Rabbit Polyclonal antibody. Positive IP detected in A431 cells. Positive WB detected in HL-60 cells, A431 cells, HeLa cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 49 kDa

Tested applications :
ELISA, IHC, IP, WB

Species reactivity :
Human,Mouse,Rat; other species not tested.

Alternative names :
HD3 antibody; HDAC3 antibody; histone deacetylase 3 antibody; RPD3 antibody; RPD3 2 antibody; SMAP45 antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of HDAC3 recombinant protein (Accession Number: NM_003883). Purification method: Antigen affinity purified.

Clonality :
Polyclonal

Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:200-1:1000IHC: 1:20-1:200

Background :
Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the (-amino groups of lysines located near the amino termini of core histone proteins. At least 4 classes of HDAC were identified. HDAC3 is a class I HDAC. HDAC3 has histone deacetylase activity and may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. HDAC3 can also down-regulate p53 function and thus modulate cell growth and apoptosis. The gene encoding HDAC3 is regarded as a potential tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against an internal region of human HDAC3.

References :
He M, Zhang B, Wei X. HDAC4/5-HMGB1 signalling mediated by NADPH oxidase activity contributes to cerebral ischaemia/reperfusion injury. Journal of cellular and molecular medicine. 17(4):531-42. 2013. Hu X, Lu X, Liu R. Histone cross-talk connects protein phosphatase 1α (PP1α) and histone deacetylase (HDAC) pathways to regulate the functional transition of bromodomain-containing 4 (BRD4) for inducible gene expression. The Journal of biological chemistry. 289(33):23154-67. 2014. Wang X, Liu J, Zhen J. Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy. Kidney international. 86(4):712-25. 2014. Yuan H, Niu Y, Liu X, Fu L. Exercise increases the binding of MEF2A to the Cpt1b promoter in mouse skeletal muscle. Acta physiologica (Oxford, England). 212(4):283-92. 2014. Seo J, Min SK, Park HR. Expression of Histone Deacetylases HDAC1, HDAC2, HDAC3, and HDAC6 in Invasive Ductal Carcinomas of the Breast. Journal of breast cancer. 17(4):323-31. 2014. Liu RZ, Garcia E, Glubrecht DD, Poon HY, Mackey JR, Godbout R. CRABP1 is associated with a poor prognosis in breast cancer: adding to the complexity of breast cancer cell response to retinoic acid. Molecular cancer. 14:129. 2015.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
Related websites: https://www.medchemexpress.com/antibodies.html
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Author: Gardos- Channel