Name :
HDAC6 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
HDAC6 Rabbit Polyclonal antibody. Positive IP detected in K-562 cells. Positive WB detected in K-562 cells, HeLa cells, HepG2 cells, HT-1080 cells, human liver tissue, MCF7 cells. Positive IHC detected in human testis tissue. Observed molecular weight by Western-blot: 160kd
Tested applications :
ELISA, WB, IHC, IP
Species reactivity :
Human, Mouse, Rat; other species not tested.
Alternative names :
FLJ16239 antibody; HD6 antibody; HDAC6 antibody; histone deacetylase 6 antibody; JM21 antibody; KIAA0901 antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of HDAC6 recombinant protein (Accession Number: BC013737). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:500-1:5000IHC: 1:20-1:200
Background :
Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. HDAC6 has some splicing variants such as P114(~130kd), P131(~160kd).This antibody is a rabbit polyclonal antibody raised against residues near the C terminal of human HDAC6.
References :
Wang X, Liu J, Zhen J. Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy. Kidney international. 86(4):712-25. 2014. Cai M, Hu Z, Liu J. Expression of hMOF in different ovarian tissues and its effects on ovarian cancer prognosis. Oncology reports. 33(2):685-92. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
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