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Name :
Anti-Biotinidase / biotinase / BTD antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
Rabbit monoclonal to Biotinidase / biotinase / BTD

Tested applications :
ELISA, IHC-P

Species reactivity :
Human Biotinidase / biotinase / BTD

Alternative names :
Biotinidase antibody; Btd antibody; biotinase antibody

Immunogen :
Human Biotinidase / biotinase / BTD (His Tag) recombinant protein

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained from a rabbit immunized with purified, recombinant Human Biotinidase / biotinase / BTD (rh Biotinidase / biotinase / BTD; P43251; Met1-Asp543).

Clonality :
Monoclonal

Formulation :
0.2 μm filtered solution in PBS

Storage instructions :
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Applications :
ELISA: 0.1-0.2 μg/mLThis antibody can be used at 0.1-0.2 μg/mL μg/ml with the appropriate secondary reagents to detect Human Biotinidase / biotinase / BTD.IHC-P: 1-10 μg/mL

Background :
Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing of fats, carbohydrates, and proteins. Biotin is attached to these carboxylase enzymes through an amino acid (the building material of proteins) called lysine, forming a complex calledbiocytin.

References :

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
Related websites: https://www.medchemexpress.com/antibodies.html
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Author: Gardos- Channel