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Name :
IFITM1-Specific antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
IFITM1-Specific Mouse Monoclonal antibody. Positive IHC detected in human lymphoma tissue. Positive WB detected in K562 cells, HeLa cells, K-562 cells. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 14kd; 17 kDa

Tested applications :
ELISA, WB, IHC, IP

Species reactivity :
Human; other species not tested.

Alternative names :
9 27 antibody; CD225 antibody; IFI17 antibody; IFITM1 antibody; Interferon induced protein 17 antibody; Leu 13 antigen antibody; LEU13 antibody

Immunogen :

Isotype :
Mouse IgG2a

Preparation :
This antibody was obtained by immunization of IFITM1-Specific recombinant protein (Accession Number: NM_003641). Purification method: Caprylic acid/ammonium sulfate precipitation.

Clonality :
Monoclonal

Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:200-1:2000IHC: 1:20-1:200

Background :
IFITM1(interferon induced transmembrane protein), also named DSPA2a and interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. 60074-1-Ig is a mouse monoclonal antibody which specifically recognizing IFITM1 but not IFITM2 or IFITM3.

References :
Zhang W, Zhang L, Zan Y, Du N, Yang Y, Tien P. Human respiratory syncytial virus infection is inhibited by IFN-induced transmembrane proteins. The Journal of general virology. 96(Pt 1):170-82. 2015. Tartour K, Appourchaux R, Gaillard J. IFITM proteins are incorporated onto HIV-1 virion particles and negatively imprint their infectivity. Retrovirology. 11:103. 2014. Xie M, Xuan B, Shan J. Human cytomegalovirus exploits interferon-induced transmembrane proteins to facilitate morphogenesis of the virion assembly compartment. Journal of virology. 89(6):3049-61. 2015. Compton AA, Bruel T, Porrot F. IFITM proteins incorporated into HIV-1 virions impair viral fusion and spread. Cell host & microbe. 16(6):736-47. 2014. Brass AL, Huang IC, Benita Y. The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus. Cell. 139(7):1243-54. 2009. Lin TY, Chin CR, Everitt AR. Amphotericin B increases influenza A virus infection by preventing IFITM3-mediated restriction. Cell reports. 5(4):895-908. 2013. Gao F, Liang H, Lu H. Global analysis of DNA methylation in hepatocellular carcinoma by a liquid hybridization capture-based bisulfite sequencing approach. Clinical epigenetics. 7(1):86. 2015. Lau DT, Negash A, Chen J. Innate immune tolerance and the role of kupffer cells in differential responses to interferon therapy among patients with HCV genotype 1 infection. Gastroenterology. 144(2):402-413.e12. 2013.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
Related websites: https://www.medchemexpress.com/antibodies.html
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Author: Gardos- Channel