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Name :
IFT140 antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
IFT140 Rabbit Polyclonal antibody. Positive IP detected in rat testis tissue. Positive WB detected in mouse testis tissue, HepG2 cells, rat testis tissue. Positive IF detected in hTERT-RPE1 cells. Observed molecular weight by Western-blot: 140 kDa

Tested applications :
ELISA, IF, WB, IP

Species reactivity :
Human,Mouse,Rat; other species not tested.

Alternative names :
c305C8.4 antibody; c380F5.1 antibody; DKFZp564L232 antibody; FLJ10306 antibody; FLJ30571 antibody; gs114 antibody; IFT140 antibody; KIAA0590 antibody; WDTC2 antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of IFT140 recombinant protein (Accession Number: NM_014714). Purification method: Antigen affinity purified.

Clonality :
Polyclonal

Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:200-1:1000IP: 1:200-1:1000IF: 1:20-1:200

Background :
IFT140 represents intraflagellar transport 140 homolog (Chlamydomonas)

References :
Miller KA, Ah-Cann CJ, Welfare MF. Cauli: a mouse strain with an Ift140 mutation that results in a skeletal ciliopathy modelling Jeune syndrome. PLoS genetics. 9(8):e1003746. 2013. Halbritter J, Bizet AA, Schmidts M. Defects in the IFT-B component IFT172 cause Jeune and Mainzer-Saldino syndromes in humans. American journal of human genetics. 93(5):915-25. 2013. Wei Q, Xu Q, Zhang Y. Transition fibre protein FBF1 is required for the ciliary entry of assembled intraflagellar transport complexes. Nature communications. 4:2750. 2013. Yang Y, Ran J, Liu M. CYLD mediates ciliogenesis in multiple organs by deubiquitinating Cep70 and inactivating HDAC6. Cell research. 24(11):1342-53. 2014. Martin CA, Ahmad I, Klingseisen A. Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy. Nature genetics. 46(12):1283-92. 2014. Xu Y, Cao J, Huang S. Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function. PloS one. 10(4):e0124378. 2015. Ran J, Yang Y, Li D, Liu M, Zhou J. Deacetylation of α-tubulin and cortactin is required for HDAC6 to trigger ciliary disassembly. Scientific reports. 5:12917. 2015. Siller SS, Burke MC, Li FQ, Takemaru K. Chibby functions to preserve normal ciliary morphology through the regulation of intraflagellar transport in airway ciliated cells. Cell cycle (Georgetown, Tex.). 14(19):3163-72. 2015.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
Related websites: https://www.medchemexpress.com/antibodies.html
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Author: Gardos- Channel