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Name :
IGFBP3 antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
IGFBP3 Rabbit Polyclonal antibody. Positive WB detected in A2780 cells, HeLa cells. Positive IF detected in HeLa cells. Positive IHC detected in human breast cancer tissue, human colon cancer tissue. Observed molecular weight by Western-blot: 37-45 kDa

Tested applications :
ELISA, WB, IHC, IF

Species reactivity :
Human, Mouse; other species not tested.

Alternative names :
BP 53 antibody; IBP 3 antibody; IBP3 antibody; IGF binding protein 3 antibody; IGFBP 3 antibody; IGFBP3 antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of IGFBP3 recombinant protein (Accession Number: XM_017012152). Purification method: Antigen affinity purified.

Clonality :
Polyclonal

Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:200-1:1000IHC: 1:20-1:200IF: 1:10-1:100

Background :
Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid.

References :

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
Related websites: https://www.medchemexpress.com/antibodies.html
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Author: Gardos- Channel