Name :
INPP5E antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
INPP5E Rabbit Polyclonal antibody. Positive WB detected in SH-SY5Y cells, HEK-293 cells, mouse brain tissue, mouse testis tissue. Positive IP detected in HEK-293 cells. Positive IF detected in hTERT-RPE1 cells. Positive IHC detected in human testis tissue, human brain tissue, human heart tissue, human kidney tissue, human ovary tissue, human placenta tissue, human spleen tissue. Observed molecular weight by Western-blot: 64-66 kDa
Tested applications :
ELISA, WB, IHC, IF, IP
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
INPP5E antibody; PPI5PIV antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of INPP5E recombinant protein (Accession Number: NM_019892). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:500-1:5000IHC: 1:20-1:200IF: 1:20-1:200
Background :
INPP5E(72 kDa inositol polyphosphate 5-phosphatase) converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2.In mouse, highest protein expression was in brain, heart, and testis, with lower expression in thymus and lung, and very little expression in kidney, spleen, and liver(PMID:10764818). Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) and mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS)(PMID:19668215). It has 2 isoforms with the molecular mass of 66 kDa and 70 kDa.
References :
Humbert MC, Weihbrecht K, Searby CC. ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting. Proceedings of the National Academy of Sciences of the United States of America. 109(48):19691-6. 2012. Plotnikova OV, Seo S, Cottle DL. INPP5E interacts with AURKA, linking phosphoinositide signaling to primary cilium stability. Journal of cell science. 128(2):364-72. 2015. Yang AW, Sachs AJ, Nystuen AM. Deletion of Inpp5a causes ataxia and cerebellar degeneration in mice. Neurogenetics. 16(4):277-85. 2015. Slaats GG, Isabella CR, Kroes HY. MKS1 regulates ciliary INPP5E levels in Joubert syndrome. Journal of medical genetics. 53(1):62-72. 2016. Thomas S, Wright KJ, Le Corre S. A homozygous PDE6D mutation in Joubert syndrome impairs targeting of farnesylated INPP5E protein to the primary cilium. Human mutation. 35(1):137-46. 2014.
Related websites: https://www.medchemexpress.com/antibodies.html
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