Name :
IRAK1 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
IRAK1 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue, human lung cancer tissue. Positive IF detected in A549 cells. Positive FC detected in HeLa cells. Positive WB detected in A549 cells, HeLa cells, PC-13 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 68-80 kDa
Tested applications :
ELISA, WB, IP, IHC, IF, FC
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
IRAK antibody; IRAK 1 antibody; IRAK1 antibody; pelle antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of IRAK1 recombinant protein (Accession Number: NM_001025243). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:200-1:2000IP: 1:200-1:2000IHC: 1:50-1:500IF: 1:50-1:500
Background :
Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)
References :
Jiang M, Xiang Y, Wang D. Dysregulated expression of miR-146a contributes to age-related dysfunction of macrophages. Aging cell. 11(1):29-40. 2012. Sun Y, Varambally S, Maher CA. Targeting of microRNA-142-3p in dendritic cells regulates endotoxin-induced mortality. Blood. 117(23):6172-83. 2011.
Related websites: https://www.medchemexpress.com/antibodies.html
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