Name :
IRF3 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
IRF3 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, human brain tissue, mouse brain tissue, rat brain tissue, U-937 cells. Positive IP detected in Jurkat cells. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human spleen tissue. Observed molecular weight by Western-blot: 49-55 kDa
Tested applications :
ELISA, WB, IHC, IF, IP, FC
Species reactivity :
Human, Mouse, Rat; other species not tested.
Alternative names :
interferon regulatory factor 3 antibody; IRF 3 antibody; IRF3 antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of IRF3 recombinant protein (Accession Number: XM_006723198). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:500-1:5000IHC: 1:20-1:200IF: 1:10-1:100
Background :
The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes
References :
Zhu M, Fang T, Li S, Meng K, Guo D. Bipartite Nuclear Localization Signal Controls Nuclear Import and DNA-Binding Activity of IFN Regulatory Factor 3. Journal of immunology (Baltimore, Md. : 1950). 195(1):289-97. 2015. Li L, Qin JJ, Guo S. Attenuation of Cerebral Ischemic Injury in Interferon Regulatory Factor 3 Deficient Rat. Journal of neurochemistry. 2015. Li S, Zhu M, Pan R. The tumor suppressor PTEN has a critical role in antiviral innate immunity. Nature immunology. 17(3):241-9. 2016. Zhu H, Zheng C, Xing J. Varicella-zoster virus immediate-early protein ORF61 abrogates the IRF3-mediated innate immune response through degradation of activated IRF3. Journal of virology. 85(21):11079-89. 2011. Zhou P, Li H, Wang H, Wang LF, Shi Z. Bat severe acute respiratory syndrome-like coronavirus ORF3b homologues display different interferon antagonist activities. The Journal of general virology. 93(Pt 2):275-81. 2012. Zhang Z, Zheng Z, Luo H. Human bocavirus NP1 inhibits IFN-β production by blocking association of IFN regulatory factor 3 with IFNB promoter. Journal of immunology (Baltimore, Md. : 1950). 189(3):1144-53. 2012. Guo R, Li Y, Ning J, Sun D, Lin L, Liu X. HnRNP A1/A2 and SF2/ASF regulate alternative splicing of interferon regulatory factor-3 and affect immunomodulatory functions in human non-small cell lung cancer cells. PloS one. 8(4):e62729. 2013. Luo H, Zhang Z, Zheng Z. Human bocavirus VP2 upregulates IFN-β pathway by inhibiting ring finger protein 125-mediated ubiquitination of retinoic acid-inducible gene-I. Journal of immunology (Baltimore, Md. : 1950). 191(2):660-9. 2013.
Related websites: https://www.medchemexpress.com/antibodies.html
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