Name :
JNK antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
JNK Mouse Monoclonal antibody. Positive IHC detected in human prostate cancer tissue. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells, C6 cells, Ethacrynic acid treated HeLa cells, HEK-293 cells, Jurkat cells. Observed molecular weight by Western-blot: 44 kDa, 55 kDa
Tested applications :
ELISA, WB, IF, IHC
Species reactivity :
Human, Mouse, Rat; other species not tested.
Alternative names :
c Jun N terminal kinase 1 antibody; JNK antibody; JNK 46 antibody; JNK1 antibody; JNK1A2 antibody; JNK21B1/2 antibody; MAP kinase 8 antibody; MAPK 8 antibody; MAPK8 antibody; PRKM8 antibody; SAPK1 antibody
Immunogen :
Isotype :
Mouse IgG1
Preparation :
This antibody was obtained by immunization of JNK recombinant protein (Accession Number: NM_001323330). Purification method: Protein G purified.
Clonality :
Monoclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IHC: 1:20-1:200IF: 1:20-1:200
Background :
MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. MAPK8 functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. It has 4 isoforms produced by alternative splicing with the molecular weight of 46 kDa and 48 kDa. This protein can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot(PMID:11062067 ).
References :
Related websites: https://www.medchemexpress.com/antibodies.html
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