Name :
Anti-Bruton Tyrosine Kinase / BTK Kinase antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
Rabbit polyclonal to Bruton Tyrosine Kinase / BTK Kinase
Tested applications :
WB, IHC-P, IP
Species reactivity :
Human Bruton Tyrosine Kinase / BTK Kinase
Alternative names :
BPK antibody; MGC126261 antibody; MGC126262 antibody; PSCTK1 antibody; RP1-164F3.2 antibody; RP23-91G19.5 antibody; xid antibody; AI528679 antibody; AT antibody; ATK antibody; BPK antibody; XLA antibody; IMD1 antibody; AGMX1 antibody; PSCTK1 antibody; AGMX1 antibody; AI528679 antibody; AT antibody; ATK antibody; BTK antibody; Btk antibody; IMD1 antibody; xid antibody; XLA antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
Produced in rabbits immunized with purified, recombinant Human Bruton Tyrosine Kinase / BTK Kinase (rh Bruton Tyrosine Kinase / BTK Kinase; NP_000052.1; Met1-Ser659). Bruton Tyrosine Kinase / BTK Kinase specific IgG was purified by Human Bruton Tyrosine Kinase / BTK Kinase affinity chromatography.
Clonality :
Polyclonal
Formulation :
0.2 μm filtered solution in PBS
Storage instructions :
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Applications :
WB: 5-10 μg/mLIHC-P: 0.1-2 μg/mLIP: 1-4 μg/mg of lysate
Background :
Bruton’s tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol(3,4,5)-trisphosphate (PIP3). After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia(Bruton’s agammaglobulinemia). BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation.
References :
Hashimoto S, et al. (1996) Identification of Bruton’s tyrosine kinase (Btk) gene mutations and characterization of the derived proteins in 35 X-linked agammaglobulinemia families: a nationwide study of Btk deficiency in Japan. Blood. 88(2): 561-73. Ohta Y, et al. (1994) Genomic organization and structure of Bruton agammaglobulinemia tyrosine kinase: localization of mutations associated with varied clinical presentations and course in X chromosome-linked agammaglobulinemia. PNAS. 91(19): 9062-6. Smith C, et al. (1994) Expression of Bruton’s agammaglobulinemia tyrosine kinase gene, BTK, is selectively down-regulated in T lymphocytes and plasma cells. The Journal of Immunology. 152(2): 557-65.
Related websites: https://www.medchemexpress.com/antibodies.html
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