Name :
KIF3A antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
KIF3A Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in human cerebellum tissue, mouse brain tissue, mouse cerebellum tissue. Positive IHC detected in human gliomas tissue. Observed molecular weight by Western-blot: 80kd
Tested applications :
ELISA, WB, IHC, IP
Species reactivity :
Human, Mouse; other species not tested.
Alternative names :
KIF3 antibody; KIF3A antibody; kinesin family member 3A antibody; Kinesin like protein KIF3A antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of KIF3A recombinant protein (Accession Number: NM_001300792). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:200-1:2000IP: 1:200-1:2000IHC: 1:20-1:200
Background :
KIF3A is a subunit of Kinesin II that is essential for intraflagellar transport (IFT). It functions as the anterograde motor to deliver ciliary components to the tip where the axoneme is assembled. Disruption of KIF3A has been reported to block mammalian ciliogenesis. Recently KIF3A has been found to localize to the mother centriole and is required for centrioles organization. This antibody specifically recognizes the endogenous KIF3A protein in mouse embryonic fibroblasts (MEFs) but not in KIF3A-/- lysate. (23386061)
References :
Kodani A, Salomé Sirerol-Piquer M, Seol A, Garcia-Verdugo JM, Reiter JF. Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages. The EMBO journal. 32(4):597-607. 2013. Guadiana SM, Semple-Rowland S, Daroszewski D. Arborization of dendrites by developing neocortical neurons is dependent on primary cilia and type 3 adenylyl cyclase. The Journal of neuroscience : the official journal of the Society for Neuroscience. 33(6):2626-38. 2013.
Related websites: https://www.medchemexpress.com/antibodies.html
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