Name :
LIMK2 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
LIMK2 Rabbit Polyclonal antibody. Positive WB detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 70-80 kDa
Tested applications :
ELISA, WB
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
LIM domain kinase 2 antibody; LIMK 2 antibody; LIMK2 antibody
Immunogen :
Human LIMK2 Recombinant protein (His tag)
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of LIMK2 recombinant protein (Accession Number: NM_001031801). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:200-1:2000
Background :
The family of LIM kinase (LIMK) includes two similar proteins, LIMK1 and LIMK2, that share overall identity of 53% with 75%, 55%, and 42% amino acid identity in the LIM, kinase, and PDZ domains, respectively. LIMK2 regulates actin cytoskeletal reorganization through phosphorylating and inactivating cofilin, an actin-depolymerizing factor of actin filaments. It also may associate with gamma-tubulin and play a role in mitotic spindle assembly. High levels of LIMK2 are evident in uterus, thymus, and spleen whereas lower levels are observed in brain, heart, liver, stomach, skeletal muscle, and testes according to the results of western blotting(PMID:16399995). It has 3 isoforms produced by alternative splicing of the molecular weight of 70-78 kDa. It can be detected a band of 47 kDa or 50-55 kDa in the testes because the antibody is recognizing the testis-specific variant of LIMK2 and the this difference could be due to species variation(PMID:17928627).
References :
Choi HJ, Sanders TA, Tormos KV. ECM-dependent HIF induction directs trophoblast stem cell fate via LIMK1-mediated cytoskeletal rearrangement. PloS one. 8(2):e56949. 2013.
Related websites: https://www.medchemexpress.com/antibodies.html
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