Name :
MAT1A antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
MAT1A Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, arabidopsis whole plant, HepG2 cells. Positive IHC detected in human lung tissue. Observed molecular weight by Western-blot: 44-55kd
Tested applications :
ELISA, WB, IHC
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
AdoMet synthase 1 antibody; AMS1 antibody; MAT antibody; MAT 1 antibody; MAT I/III antibody; MAT1A antibody; MATA1 antibody; SAMS antibody; SAMS1 antibody
Immunogen :
Human MAT1A Recombinant protein (GST tag)
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of MAT1A recombinant protein (Accession Number: XM_005269842). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:1000-1:10000IHC: 1:20-1:200
Background :
Methionine adenosyltransferase (MAT) is the enzyme that catalyzes the synthesis of S-adeno-sylmethionine (AdoMet), the main donor of methyl groups in the cell. In mammals MAT is encoded by two different genes, MAT1A and MAT2A. MAT1A is expressed only in the mature liver whereas fetal hepatocytes, extrahepatic tissues and liver cancer cells express MAT2A. This antibody was raised against the full length of human MAT1A and may cross-react with MAT2A. Western blot analysis using this antibody detected a single band around 44-50kD in liver.
References :
Li J, Ramani K, Sun Z. Forced expression of methionine adenosyltransferase 1A in human hepatoma cells suppresses in vivo tumorigenicity in mice. The American journal of pathology. 176(5):2456-66. 2010. Thomas A, Stevens AP, Klein MS. Early changes in the liver-soluble proteome from mice fed a nonalcoholic steatohepatitis inducing diet. Proteomics. 12(9):1437-51. 2012. Vitvitsky V, Martinov M, Ataullakhanov F, Miller RA, Banerjee R. Sulfur-based redox alterations in long-lived Snell dwarf mice. Mechanisms of ageing and development. 134(7-8):321-30. 2013. Chen M, Zhou K, Chen X. Metabolomic analysis reveals metabolic changes caused by bisphenol A in rats. Toxicological sciences : an official journal of the Society of Toxicology. 138(2):256-67. 2014. Brown-Borg HM, Rakoczy S, Wonderlich JA, Armstrong V, Rojanathammanee L. Altered dietary methionine differentially impacts glutathione and methionine metabolism in long-living growth hormone-deficient Ames dwarf and wild-type mice. Longevity & healthspan. 3(1):10. 2014.
Related websites: https://www.medchemexpress.com/antibodies.html
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