Name :
acetylated Tubulin (Lys40) antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
acetylated Tubulin (Lys40) Mouse Monoclonal antibody. Positive IF detected in MDCK cells. Positive IHC detected in human lung cancer tissue, human gliomas tissue. Positive WB detected in L02 cells. Observed molecular weight by Western-blot: 55 kDa
Tested applications :
ELISA, WB, IHC, IF
Species reactivity :
Human, Mouse, Rat, Dog; other species not tested.
Alternative names :
acetylated alpha tubulin antibody; Acetylated tubulin antibody; acetylated-a-tubulin antibody; acetyl-a-tubulin antibody; alpha tubulin antibody; Alpha tubulin 3 antibody; tubulin antibody
Immunogen :
Isotype :
Mouse IgG1
Preparation :
This antibody was obtained by immunization of Peptide (Accession Number: NM_006009). Purification method: Protein G purified.
Clonality :
Monoclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IHC: 1:20-1:200IF: 1:20-1:200
Background :
Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin. (24268707, 23881549)
References :
Li L, Liu B, Zhang X, Ye L. The oncoprotein HBXIP promotes migration of breast cancer cells via GCN5-mediated microtubule acetylation. Biochemical and biophysical research communications. 458(3):720-5. 2015. Chen T, Wu Q, Zhang Y, Zhang D. NDUFV2 regulates neuronal migration in the developing cerebral cortex through modulation of the multipolar-bipolar transition. Brain research. 1625:102-10. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
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