Name :
MCL1 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
MCL1 Mouse Monoclonal antibody. Positive IHC detected in human breast cancer tissue. Positive IF detected in MCF-7 cells, Hela cells. Positive WB detected in Hela cells, A431 cells, Jurkat cells. Observed molecular weight by Western-blot: 40 kDa
Tested applications :
ELISA, WB, IF, IHC
Species reactivity :
Human; other species not tested.
Alternative names :
EAT antibody; Mcl 1 antibody; Mcl 1l antibody; Mcl 1s Delta TM antibody; MCL1 antibody; mcl1/EAT antibody; MCL1L antibody; MCL1S antibody
Immunogen :
Isotype :
Mouse IgG2b
Preparation :
This antibody was obtained by immunization of MCL1 recombinant protein (Accession Number: NM_021960). Purification method: Protein A purified.
Clonality :
Monoclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IHC: 1:20-1:200IF: 1:10-1:100
Background :
MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target.
References :
Jiang W, Wang Z, Li X. Reduced high-mobility group box 1 expression induced by RNA interference inhibits the bioactivity of hepatocellular carcinoma cell line HCCLM3. Digestive diseases and sciences. 57(1):92-8. 2012. Sun Y, Liu Z, Zou X. Mechanisms underlying 3-bromopyruvate-induced cell death in colon cancer. Journal of bioenergetics and biomembranes. 47(4):319-29. 2015. Hu H, Tang C, Jiang Q. Reduced ubiquitin-specific protease 9X expression induced by RNA interference inhibits the bioactivity of hepatocellular carcinoma cells. Oncology letters. 10(1):268-272. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
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