Name :
ME1 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
ME1 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue, human placenta tissue. Positive WB detected in mouse liver tissue, mouse placenta tissue. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 55-64kd
Tested applications :
ELISA, WB, IHC, IP
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
HUMNDME antibody; Malic enzyme 1 antibody; ME1 antibody; MES antibody; NADP dependent malic enzyme antibody; NADP ME antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of ME1 recombinant protein (Accession Number: NM_002395). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:200-1:2000IHC: 1:20-1:200
Background :
ME1(NADP-dependent malic enzyme) belongs to the malic enzymes family. This malic enzyme catalyzes the reversible oxidative decarboxylation of malate and is a link between the glycolytic pathway and the citric acid cycle. The reaction is L-malate plus NADP(+) to form pyruvate, CO(2), and NADPH. The predicted protein contains 572 amino acids and has a calculated molecular mass of 64.1 kDa.
References :
Peinado JR, Quirós PM, Pulido MR. Proteomic profiling of adipose tissue from Zmpste24-/- mice, a model of lipodystrophy and premature aging, reveals major changes in mitochondrial function and vimentin processing. Molecular & cellular proteomics : MCP. 10(11):M111.008094. 2011. Al-Dwairi A, Pabona JM, Simmen RC, Simmen FA. Cytosolic malic enzyme 1 (ME1) mediates high fat diet-induced adiposity, endocrine profile, and gastrointestinal tract proliferation-associated biomarkers in male mice. PloS one. 7(10):e46716. 2012. Mitsuishi Y, Taguchi K, Kawatani Y. Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming. Cancer cell. 22(1):66-79. 2012. Al-Dwairi A, Brown AR, Pabona JM. Enhanced gastrointestinal expression of cytosolic malic enzyme (ME1) induces intestinal and liver lipogenic gene expression and intestinal cell proliferation in mice. PloS one. 9(11):e113058. 2014. Sun L, Song L, Wan Q. cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. Cell research. 25(4):429-44. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
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