Share this post on:

Name :
MECP2 antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
MECP2 Rabbit Polyclonal antibody. Positive IP detected in MCF-7 cells. Positive WB detected in MDA-MB-453s cells, human brain tissue, mouse heart tissue, mouse lung tissue, mouse ovary tissue, SH-SY5Y cells. Positive IF detected in HepG2 cells. Positive IHC detected in human prostate cancer tissue, human brain tissue. Positive FC detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 75 kDa

Tested applications :
ELISA, IHC, WB, IF, IP, FC

Species reactivity :
Human,Mouse,Rat; other species not tested.

Alternative names :
AUTSX3 antibody; DKFZp686A24160 antibody; MeCp 2 protein antibody; MECP2 antibody; Methyl CpG binding protein 2 antibody; MRX16 antibody; MRX79 antibody; MRXS13 antibody; MRXSL antibody; PPMX antibody; RTS antibody; RTT antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of MECP2 recombinant protein (Accession Number: NM_004992). Purification method: Antigen affinity purified.

Clonality :
Polyclonal

Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:200-1:2000IP: 1:200-1:2000IHC: 1:20-1:200IF: 1:20-1:200

Background :
METHYL-CpG-BINDING PROTEIN 2(MECP2), is a chromatin-associated protein that can both activate and repress transcription. Mecp2 in the adult mouse is high in the brain, lung, and spleen, lower in heart and kidney. MECP2 takes part in the control of neuronal activity-dependent gene regulation, and this process may underlie the pathology of Rett syndrome, a severe developmental disorder with autistic phenotypes. As compared to wild-type monkeys, MECP2 transgenic monkeys exhibited a higher frequency of repetitive circular locomotion and increased stress responses, as measured by the threat-related anxiety and defensive test. Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Two isoforms of MECP2 exist due to alternative splicing events.This antibody reacts with the MECP2 and phosphorylated MECP2 proteins.

References :
Wang Y, Liu C, Guo QL. Intrathecal 5-azacytidine inhibits global DNA methylation and methyl- CpG-binding protein 2 expression and alleviates neuropathic pain in rats following chronic constriction injury. Brain research. 1418:64-9. 2011. Leoh LS, van Heertum B, De Rijck J. The stress oncoprotein LEDGF/p75 interacts with the methyl CpG binding protein MeCP2 and influences its transcriptional activity. Molecular cancer research : MCR. 10(3):378-91. 2012. Basu A, Woods-Burnham L, Ortiz G. Specificity of antinuclear autoantibodies recognizing the dense fine speckled nuclear pattern: Preferential targeting of DFS70/LEDGFp75 over its interacting partner MeCP2. Clinical immunology (Orlando, Fla.). 161(2):241-50. 2015.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
Related websites: https://www.medchemexpress.com/antibodies.html
Popular product recommendations:
BrdU Rabbit mAb supplier
Chk1 Rabbit pAb Epigenetic Reader Domain
TIMP2 Antibody: TIMP2 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 24 kDa, targeting to TIMP2. It can be used for WB,FC assays with tag free, in the background of Human.

Share this post on:

Author: Gardos- Channel