Name :
MED20 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
MED20 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, HeLa cells. Observed molecular weight by Western-blot: 28kd
Tested applications :
ELISA, WB
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
DKFZp586D2223 antibody; hTRFP antibody; MED20 antibody; mediator complex subunit 20 antibody; PRO0213 antibody; TRF proximal protein homolog antibody; TRFP antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of MED20 recombinant protein (Accession Number: NM_004275). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000
Background :
MED20(Mediator of RNA polymerase II transcription subunit 20) is also named as TRFP and belongs to the Mediator complex subunit 20 family. The deduced 209-amino acid protein shares 44% amino identity with Drosophila Trfp. TRFP had an apparent molecular mass of 28 kD by SDS-PAGE. It is identified TRFP as a component of an RNA polymerase II-SRB complex. The TRFP-containing complex could substitute for core RNA polymerase II in driving basal transcription from a minimal promoter.
References :
Vijayalingam S, Chinnadurai G. Adenovirus L-E1A activates transcription through mediator complex-dependent recruitment of the super elongation complex. Journal of virology. 87(6):3425-34. 2013.
Related websites: https://www.medchemexpress.com/antibodies.html
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