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Name :
MED8 antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
MED8 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, mouse liver tissue, SGC-7901 cells. Observed molecular weight by Western-blot: 29 kDa

Tested applications :
ELISA, WB

Species reactivity :
Human, Mouse; other species not tested.

Alternative names :
ARC32 antibody; MED8 antibody; mediator complex subunit 8 antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of MED8 recombinant protein (Accession Number: BC010543). Purification method: Antigen affinity purified.

Clonality :
Polyclonal

Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:500-1:5000

Background :
MED8, a component of the Mediator complex, is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. It plays a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. MED8 has three isoforms with MW 19 kDa, 29 kDa and 33 kDa. And the ubiquitination form will be 38-43 kDa.

References :

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
Related websites: https://www.medchemexpress.com/antibodies.html
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Author: Gardos- Channel