Name :
MICAL1 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
MICAL1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in Jurkat cells, HEK-293 cells, HeLa cells, human brain tissue, mouse lung tissue. Positive IF detected in HeLa. Positive IHC detected in human lung tissue, human heart tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 120 kDa
Tested applications :
ELISA, WB, IHC, IP, FC, IF
Species reactivity :
Human, Mouse; other species not tested.
Alternative names :
DKFZp434B1517 antibody; FLJ11937 antibody; FLJ21739 antibody; MICAL antibody; MICAL 1 antibody; MICAL1 antibody; NICAL antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of MICAL1 recombinant protein (Accession Number: NM_022765). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:1000-1:10000IP: 1:500-1:5000IHC: 1:20-1:200IF: 1:50-1:500
Background :
MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL1 has four conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, a Lin11, Isl-1 and Mec-3 (LIM) domain and a C-terminal coiled-coil (CC) domain. MICAL1 is reported to regulate actin stress fibers and be required for normal actin organization. It may also be involved in apoptosis through binding with NDR (nuclear Dbf2-related) kinases. This antibody specially recognizes MICAL1.
References :
Giridharan SS, Rohn JL, Naslavsky N, Caplan S. Differential regulation of actin microfilaments by human MICAL proteins. Journal of cell science. 125(Pt 3):614-24. 2012. Aggarwal PK, Veron D, Thomas DB. Semaphorin3a promotes advanced diabetic nephropathy. Diabetes. 64(5):1743-59. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
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