Name :
MIPEP antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
MIPEP Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, MCF7 cells. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 70-80 kDa
Tested applications :
ELISA, IHC, WB
Species reactivity :
Human; other species not tested.
Alternative names :
HMIP antibody; MIP antibody; MIPEP antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of MIPEP recombinant protein (Accession Number: NM_005932). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IHC: 1:20-1:200
Background :
MIPEP(Mitochondrial intermediate peptidase) is also named as MIP and belongs to the peptidase M3 family. It performs the final step in processing a specific class of nuclear-encoded proteins targeted to the mitochondrial matrix or inner membrane. The enzyme is a monomer of 75 kDa with a transit peptide and about 20% of the fmal MIP preparation consists of equimolar amounts of two peptides of 47 kDa and 28 kDa, which are apparently the products of a single cleavage of the 75 kDa protein. MIP is an independent endopeptidase acting on mitochondrial intermediate proteins as substrates.(PMID:1322290).
References :
Marcondes MF, Torquato RJ, Assis DM, Juliano MA, Hayashi MA, Oliveira V. Mitochondrial intermediate peptidase: expression in Escherichia coli and improvement of its enzymatic activity detection with FRET substrates. Biochemical and biophysical research communications. 391(1):123-8. 2010.
Related websites: https://www.medchemexpress.com/antibodies.html
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