Name :
Anti-Carbonic Anhydrase IX/CA9 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
Rabbit polyclonal to Carbonic Anhydrase IX/CA9
Tested applications :
ELISA, WB, IP
Species reactivity :
Canine Carbonic Anhydrase IX / CA9
Alternative names :
Car9 antibody; Carbonic Anhydrase 9 antibody; Carbonic Anhydrase IX antibody; Carbonic Anhydrase IX antibody; MN/CA9 antibody; RP23-195K8.9 antibody; MN antibody; CA9 antibody; CAIX antibody; CAIX antibody
Immunogen :
Canine Carbonic Anhydrase IX/CA9 (His Tag) recombinant protein
Isotype :
Rabbit IgG
Preparation :
Produced in rabbits immunized with purified, recombinant Canine Carbonic Anhydrase IX / CA9 (rh Carbonic Anhydrase IX / CA9; NP_001138646.1; Met1-Leu410). Carbonic Anhydrase IX / CA9 specific IgG was purified by Canine Carbonic Anhydrase IX / CA9 affinity chromatography.
Clonality :
Polyclonal
Formulation :
0.2 μm filtered solution in PBS
Storage instructions :
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Applications :
WB: 10-50 μg/mLELISA: 0.1-0.2 μg/mLThis antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Canine Carbonic Anhydrase IX / CA9. The detection limit for Canine Carbonic Anhydrase IX / CA9 is IP: 4-6 μg/mg of lysate
Background :
Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.
References :
Loncaster JA, et al. (2001) Carbonic anhydrase (CA IX) expression, a potential new intrinsic marker of hypoxia: correlations with tumor oxygen measurements and prognosis in locally advanced carcinoma of the cervix. Cancer Res. 61(17): 6394-9. Zvada J, et al. (2003) Soluble form of carbonic anhydrase IX (CA IX) in the serum and urine of renal carcinoma patients. Br J Cancer. 89(6): 1067-71. Pan P, et al. (2006) Carbonic anhydrase gene expression in CA II-deficient (Car2-/-) and CA IX-deficient (Car9-/-) mice. J Physiol. 571(Pt 2): 319-27. Zhou GX, et al. (2010) Quantification of carbonic anhydrase IX expression in serum and tissue of renal cell carcinoma patients using enzyme-linked immunosorbent assay: prognostic and diagnostic potentials. Urology. 75(2): 257-61.
Related websites: https://www.medchemexpress.com/antibodies.html
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