Name :
MOCS3 Polyclonal Antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
Polyclonal antibody to MOCS3
Tested applications :
WB
Species reactivity :
Human, Mouse, Rat
Alternative names :
MOCS3 antibody; UBA4 antibody; molybdenum cofactor synthesis 3 antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 271-460 of human MOCS3 (NP_055299.1).
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage instructions :
Store at -20℃. Avoid freeze / thaw cycles.
Applications :
WB 1:500 – 1:2000
Background :
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.
References :
Related websites: https://www.medchemexpress.com/antibodies.html
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