Name :
Anti-Carbonic Anhydrase X antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
Rabbit polyclonal to Carbonic Anhydrase X
Tested applications :
ELISA, WB
Species reactivity :
Mouse Carbonic Anhydrase X / CA10
Alternative names :
2700029L05Rik antibody; BB085816 antibody; CA10 antibody; Car10 antibody; Carbonic Anhydrase 10 antibody; Carbonic Anhydrase X antibody; CARPX antibody; CA-RPX antibody; HUCEP-15 antibody; RP23-448I9.1 antibody
Immunogen :
Mouse Carbonic Anhydrase X (His Tag) recombinant protein
Isotype :
Rabbit IgG
Preparation :
Produced in rabbits immunized with purified, recombinant Mouse Carbonic Anhydrase X / CA10 (rM Carbonic Anhydrase X / CA10; P61215; Met1-Asn300). Carbonic Anhydrase X / CA10 specific IgG was purified by Mouse Carbonic Anhydrase X / CA10 affinity chromatography.
Clonality :
Polyclonal
Formulation :
0.2 μm filtered solution in PBS with 5% trehalose
Storage instructions :
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Applications :
WB: 5-10 μg/mLELISA: 0.1-0.2 μg/mLThis antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse Carbonic Anhydrase X / CA10. The detection limit for Mouse Carbonic Anhydrase X / CA10 is approximately 0.039 ng/well.
Background :
Carbonic anhydrase X, also called carbonic anhydrase – related protein X (CARPX) and CA10, belongs to the CA family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. The secreted protein CARPX without CA activity (hydration of CO2) is identified as an acatalytic member of the alpha-carbonic anhydrase subgroup. CARP X expression is detected in the adult total brain and almost all parts of the central nervous system, but not in the fetal brain. Accordingly, CARP X is suggested to play a role in the development of central nervous system, especially the brain. The same CARP X protein are encoded by multiple transcript variants of this gene.
References :
Okamoto, N. et al., 2001, Biochim. Biophys. Acta 1518:311-316. Taniuchi, K. et al., Neuroscience 112: 93-99. Supuran, C.T. et al., 2003, Med. Res. Rev. 23: 146-189.
Related websites: https://www.medchemexpress.com/antibodies.html
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