Name :
Anti-Carbonic Anhydrase XII antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
Rabbit polyclonal to Carbonic Anhydrase XII
Tested applications :
ELISA, IHC-P
Species reactivity :
Mouse Ca12 / Car12
Alternative names :
2310047E01Rik antibody; AI314958 antibody; CA12 antibody; Car12 antibody; Carbonic Anhydrase 12 antibody; Carbonic Anhydrase XII antibody; Carbonic Anhydrase XII antibody; CAXII antibody; FLJ20151 antibody; HsT18816 antibody
Immunogen :
Mouse Carbonic Anhydrase XII (His Tag) recombinant protein
Isotype :
Rabbit IgG
Preparation :
Produced in rabbits immunized with purified, recombinant Mouse Ca12 (rM Ca12; NP_848483.2; Met 1-Ser 301). Ca12 specific IgG was purified by mouse Ca12 affinity chromatography.
Clonality :
Polyclonal
Formulation :
0.2 μm filtered solution in PBS with 5% trehalose
Storage instructions :
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Applications :
ELISA: 0.5-1 μg/mLThis antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Mouse Ca12. The detection limit for Mouse Ca12 is 0.00245 ng/well.IHC-P: 0.1-2 μg/mL
Background :
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.
References :
Sahin, U. et al., 1996, Proc. Natl. Acad. Sci. U.S.A. 92 (25): 11810–11813. Ivanov, S.V. et al., 1998, Proc. Natl. Acad. Sci. USA 95:12596 – 12601. Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899 – 16903. Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 – 262. Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602. Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222.
Related websites: https://www.medchemexpress.com/antibodies.html
Popular product recommendations:
RPA32 Antibody (YA679) medchemexpress
DNMT1 Mouse mAb Epigenetics
AKT1/2/3 Antibody (YA633): AKT1/2/3 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 56 kDa, targeting to AKT1/2/3. It can be used for WB,ICC/IF,IHC-P,IP,FC assays with tag free, in the background of Human, Mouse.
