Parated and transferred onto nylon membranes (Amersham Phamacia Biotech), which were probed with 32P-labeled probes prepared according to Cai et al [36] and exposed to x-ray films.sucrose gradients. The rRNAs were detected by ethidium bromide (EtBr) staining. The size of the MNS biological activity transcript (in kb) is shown. (TIF) Polysome Association Analysis of Chloroplast Transcripts in Wild-Type and cplepa-1 Plants Grown on MS. The association of the psbA, psbB, atpB, psaA, petB and rrn23 transcripts with polysomes. Total extracts from wild-type and cplepa-1 leaves grown on MS solid medium supplied with 2 sucrose for 3 weeks under 120 mmol m22 s21 illumination were fractionated on 15 ?5 sucrose gradients. Ten fractions of equal volume were collected from the top to the bottom of the sucrose gradients, and equal proportions of the RNA purified from each fraction were analyzed by northern blot. The rRNAs were detected by ethidium bromide (EtBr) staining. The size of the transcript (in kb) is shown. (TIF)Figure SSupporting InformationFigure S1 Transmission Electron Micrographs of the Chloroplasts. Transmission electron microscopic images of the chloroplast ultrastructure in WT and cplepa-1 leaf sections. Threeweek-old plants grown on soil at 120 mmol m22 s21 were used. The scale bar indicates 1 mm. In total, 100 chloroplasts of the WT and cplepa-1 were examined and measured. (TIF) Figure SNorthern Blot Analysis for Chloroplast Transcripts in Wild-Type and cplepa-1 Plants. Northern blot analysis of chloroplast transcripts psbA, psbB, psbD, atpB, psaA, petB, rbcL, and rrn23 in wild-type and cplepa-1 mutant plants. The lanes were loaded with 10 mg total RNA each. Wild-type and cplepa-1 were grown on MS solid medium supplied with 2 sucrose for 3 weeks under 120 mmol m22 s21 illumination. Additionally, 25S rRNA stained with EtBr was loaded as a control. The size of the transcript (in kb) is shown. (TIF)Figure S4 Table S1 Primer sequences and probes used in this work.(DOC)Polysome Association Analysis for Chloroplast Transcripts in Wild-Type and cplepa-1 Plants Grown in Soil. A: The association of rrn23 and rbcL transcripts with EDTA treated polysomes. Crude leaf lysates treated with 20 mM EDTA from wild-type were size fractionated on 15 to 55 sucrose gradients containing 1 mM EDTA. B: The 24272870 association of ndhA, petA and psaJ transcripts with polysomes. Total extracts from wild-type and cplepa-1 leaves grown on soil for 3 weeks at 120 mmol m22 s21 were fractionated on 15 ?5AcknowledgmentsWe thank ABRC for the cplepa mutant lines.Author ContributionsConceived and designed the experiments: D-LJ L-XZ. Performed the experiments: D-LJ HL. Analyzed the data: D-LJ HL WC L-XZ. Contributed reagents/materials/analysis tools: D-LJ HL. Wrote the paper: 15857111 D-LJ L-XZ.
The involvement of ETS genes in cancer was first demonstrated by the presence of the oncogene v-ets as part of the gag-myb-ets transforming fusion protein of an avian retrovirus, E26 [1]. Their importance in human carcinogenesis is supported by the observations that ETS genes are implicated in chromosomal translocations, giving rise to fusion proteins that play an important role in the genesis of several hematological MedChemExpress PS 1145 malignances, soft tissue tumors and carcinomas [2]. The ETS family of transcription factors is one of the largest families of transcription regulators (27 members in the human genome), and plays an important role in diverse biological processes, including cell proliferation, apoptosis,differentiation.Parated and transferred onto nylon membranes (Amersham Phamacia Biotech), which were probed with 32P-labeled probes prepared according to Cai et al [36] and exposed to x-ray films.sucrose gradients. The rRNAs were detected by ethidium bromide (EtBr) staining. The size of the transcript (in kb) is shown. (TIF) Polysome Association Analysis of Chloroplast Transcripts in Wild-Type and cplepa-1 Plants Grown on MS. The association of the psbA, psbB, atpB, psaA, petB and rrn23 transcripts with polysomes. Total extracts from wild-type and cplepa-1 leaves grown on MS solid medium supplied with 2 sucrose for 3 weeks under 120 mmol m22 s21 illumination were fractionated on 15 ?5 sucrose gradients. Ten fractions of equal volume were collected from the top to the bottom of the sucrose gradients, and equal proportions of the RNA purified from each fraction were analyzed by northern blot. The rRNAs were detected by ethidium bromide (EtBr) staining. The size of the transcript (in kb) is shown. (TIF)Figure SSupporting InformationFigure S1 Transmission Electron Micrographs of the Chloroplasts. Transmission electron microscopic images of the chloroplast ultrastructure in WT and cplepa-1 leaf sections. Threeweek-old plants grown on soil at 120 mmol m22 s21 were used. The scale bar indicates 1 mm. In total, 100 chloroplasts of the WT and cplepa-1 were examined and measured. (TIF) Figure SNorthern Blot Analysis for Chloroplast Transcripts in Wild-Type and cplepa-1 Plants. Northern blot analysis of chloroplast transcripts psbA, psbB, psbD, atpB, psaA, petB, rbcL, and rrn23 in wild-type and cplepa-1 mutant plants. The lanes were loaded with 10 mg total RNA each. Wild-type and cplepa-1 were grown on MS solid medium supplied with 2 sucrose for 3 weeks under 120 mmol m22 s21 illumination. Additionally, 25S rRNA stained with EtBr was loaded as a control. The size of the transcript (in kb) is shown. (TIF)Figure S4 Table S1 Primer sequences and probes used in this work.(DOC)Polysome Association Analysis for Chloroplast Transcripts in Wild-Type and cplepa-1 Plants Grown in Soil. A: The association of rrn23 and rbcL transcripts with EDTA treated polysomes. Crude leaf lysates treated with 20 mM EDTA from wild-type were size fractionated on 15 to 55 sucrose gradients containing 1 mM EDTA. B: The 24272870 association of ndhA, petA and psaJ transcripts with polysomes. Total extracts from wild-type and cplepa-1 leaves grown on soil for 3 weeks at 120 mmol m22 s21 were fractionated on 15 ?5AcknowledgmentsWe thank ABRC for the cplepa mutant lines.Author ContributionsConceived and designed the experiments: D-LJ L-XZ. Performed the experiments: D-LJ HL. Analyzed the data: D-LJ HL WC L-XZ. Contributed reagents/materials/analysis tools: D-LJ HL. Wrote the paper: 15857111 D-LJ L-XZ.
The involvement of ETS genes in cancer was first demonstrated by the presence of the oncogene v-ets as part of the gag-myb-ets transforming fusion protein of an avian retrovirus, E26 [1]. Their importance in human carcinogenesis is supported by the observations that ETS genes are implicated in chromosomal translocations, giving rise to fusion proteins that play an important role in the genesis of several hematological malignances, soft tissue tumors and carcinomas [2]. The ETS family of transcription factors is one of the largest families of transcription regulators (27 members in the human genome), and plays an important role in diverse biological processes, including cell proliferation, apoptosis,differentiation.
