Ease the gel strength from the resultant cellulase could act around the algal cell wall, promoting the dissolution of agar for the duration of hot agar, cellulase could act around the algal cell wall, advertising the dissolution of agar during water extraction. Moreover, immediately after acid and bleaching treatment options, the loss rates of algae hot water extraction. In addition, right after acid and bleaching therapies, the loss rates of were 19 and 23 , respectively, which were 42 and 52 higher than these just after enzyme algae had been 19 and 23 , respectively, which were 42 and 52 higher than these aftreatment, indicating that a sizable amount of cellulose, pigment, and protein still existed in ter enzyme remedy, indicating that a large quantity of cellulose, pigment, and protein algae soon after enzyme treatment. This outcome also corresponded to the low whiteness andMar. Drugs 2021, 19,8 ofstill existed in algae after enzyme treatment. This outcome also corresponded for the low whiteness and transparency of agar just after enzymatic therapy. Despite the fact that the loss price of algae increased gradually, the yield of agar only Tenidap Technical Information changed slightly, which indicated that there was no phenomenon of agar running out just after distinct processing procedures. In the gel strength, compared with all the agar extract by untreated seaweed, the agar extracted following enzyme and bleaching therapies had a high gel strength, whereas the gel strength in the agar extracted just after acid treatment was low. This phenomenon is mainly due to the algal physique that effortlessly softens soon after acid remedy. During hot water extraction, fiber disperses in agar answer and with all the agar solution by means of the filter cloth together. This outcome is constant having a marked lower in transparency as indicated in Table 1. At present, the sulfated polysaccharides obtained from various seaweeds have attracted great interest as a result of their unique biological effects, including anticancer, antioxidant, antifungal, antiviral, and anticoagulant activities [21,22]. For instance, Liu et al. [23] discovered that the sulfated polysaccharide from G. lemaneiformis exhibited the capability to promote the function of regulatory Treg cells and alleviate allergy symptoms, which could possibly be created as a ML-SA1 Autophagy functional food component for the allergic individuals. Cui et al. [24] reported that the sulfated polysaccharides extracted from red algae have powerful anti-inflammatory activities and protected THP-1 cells against LPS-induced toxicity. Therefore, organic sulfated agar is no doubt a type of sulfated polysaccharide resource which has the potential to nutraceutical or pharmaceutical application. Nevertheless, each alkali extraction and enzymatic-assisted extraction can considerably lessen the sulfate content of agar, and direct hot water extraction cannot get high purity agar sulfated polysaccharide, so enzymatic extraction is a much more appropriate method to obtain agar sulfated polysaccharide. Soon after enzyme treatment, the contents of sulfate and 3,6-AG and gel strength of agar were not significantly various from those extracted from untreated G. lemaneiformis (Figure 5C1 ,C2 ). Even so, the weight of seaweed decreased by 10 , along with the agar yield was decreased by 9 . The loss weight of seaweed was mainly attributed to the degradation of cellulose. The reduce in the agar extraction rate was because of the broken algal cell wall conducive to the dissolution of agar throughout hot water extraction. As such, the extracted agar contained significantly less impurities. Having said that, agar from untreated G. lemaneiformis was dif.
