ved amino acid residue. G1 mom , a heterozygous carrier, presented with menorrhagia (BS = 2). Five females while in the S2 pedigree had been also heterozygous carriers with the variant but only two of these present using a bleeding diathesis. Conclusions: The GATA-1 p.His289Tyr variant resulted in mild CCR8 Agonist drug anemia, impaired platelet aggregation and secretion in hemizygous carriers. This can be the primary variant located during the GATA-1 C-terminal Zn-finger connected with platelet dysfunction and bleeding.cartridges, light transmission aggregometry, lumi-aggregometry, movement cytometry, mepacrine uptake/release assay, Prothrombin time, Activated partial thromboplastic time, Fibrinogen, Aspect Assays and Ristocetin Cofactor assay. Individuals with coagulation issue deficiency or Von Willebrand Sickness have been excluded. Patients with PFD were included, whilst patients without haemostatic defect after detailed workup(n = 120) had been taken as controls. Results: Total of 498 individuals were included out of which 67 had Bernard Soulier Syndrome(BSS), 208 had Glanzmann Thrombasthenia(GT),103 had mild PFD(storage pool defect / signal transduction defect / secretion defect) and 120 individuals with no haemostatic defect have been taken as controls. All round, CT on PFA-200 Collagen/Epinephrine had highest sensitivity(98.6 ) and negative predictive value(NPV)(96 ) as screening tool for PFD. Sensitivity and NPV of BT, PFA-200 using Collagen/PB0897|Utility of Modified Ivy’s Bleeding Time and Closure Time on Platelet Function Analyzer-200 like a Screening Tool to Identify Platelet Function Disorders R. Dave; T. Geevar; J. Mammen; G. Chellaiya; A. Samuel; R. Vijayan; S. Singh; S. Nair Christian Health-related University and Hospital, Vellore, India Background: Modified Ivy’s Bleeding time(BT) is very low price but skillbased, invasive and operator-dependent screening check for platelet function defects(PFD). Platelet Perform Analyzer-200 (PFA-200) is really a pseudo-physiological program wherein citrated entire blood is drawn at high shear through a tiny aperture in membrane coated with collagen/epinephrine or collagen/ADP, creating platelet adhesion and aggregation occluding the aperture. Time through the start off with the check right up until occlusion of your aperture will be the Closure Time(CT). Prolonged CT indicates principal haemostatic defect. Aims: To assess the functionality of modified Ivy’s BT and PFA-200 CT as screening exams for PFD. Strategies: Patients referred to our institution for bleeding workup from January 2016-January 2021 had been incorporated soon after informed consent. In depth workup was done by complete blood count, BT, PFA-200 CT applying Collagen/ADP and Collagen/Epinephrine ADP and Collagen/Epinephrine was highest(one hundred ) for identification of GT followed by BSS and least for mild PFDs.(Figure1,two) FIGURE 1 Sensitivity of Modified Ivy’s Bleeding Time, Closure Time on PFA-200 Collagen/ADP cartridge (COL/ADP) and Collagen/ Epinephrine cartridge (COL/EPI) for identification of Glanzmann Thrombasthenia (GT), Bernard Soulier Syndrome (BSS), Mild Platelet perform defects (PFD) and General platelet function disorders670 of|ABSTRACTdefects and 4 sufferers with other defects. Platelet count and Platelet Imply Volume (mean SD) in patients’ complete blood had been 27346 x 103/L and eight.7 fl, respectively. IL-8 Antagonist Purity & Documentation PFA-100 was examined in 36/50 individuals identified to get IPD of which 69 (25) gave abnormal CT. Movement cytometry final results examined on individuals with GT showed lack of expression of CD41 and CD61 on platelet surface. Conclusions: Our existing examine unveiled that se
