Lishment in budding yeast. Mol Biol Cell 21(17):3007016. 27. Wittmann JG, Rudolph MG (2004) Crystal structure of Rab9 complexed to GDP reveals a dimer with an active conformation of switch II. FEBS Lett 568(1-3):239. 28. Beck R, et al. (2008) Membrane curvature induced by Arf1-GTP is crucial for vesicle formation. Proc Natl Acad Sci USA 105(33):117311736. 29. Gasper R, Meyer S, Gotthardt K, Sirajuddin M, Wittinghofer A (2009) It takes two to tango: Regulation of G proteins by dimerization. Nat Rev Mol Cell Biol 10(6):42329. 30. G denhaupt J, et al. (2012) N-Ras types dimers at POPC membranes. Biophys J 103(7): 1585593. 31. Stokoe D, Macdonald SG, Cadwallader K, Symons M, Hancock JF (1994) Activation of Raf as a result of recruitment towards the plasma membrane. Science 264(5164):1463467. 32. Inouye K, Mizutani S, Koide H, Kaziro Y (2000) Formation in the Ras dimer is crucial for Raf-1 activation. J Biol Chem 275(six):3737740. 33. Gureasko J, et al. (2008) Membrane-dependent signal integration by the Ras activator Son of sevenless. Nat Struct Mol Biol 15(five):45261.34. Das J, et al. (2009) Digital signaling and hysteresis characterize ras activation in lymphoid cells. Cell 136(2):33751. 35. Prior IA, Muncke C, Parton RG, Hancock JF (2003) Direct visualization of Ras proteins in spatially distinct cell surface microdomains. J Cell Biol 160(2):16570. 36. Saffman PG, Delbr k M (1975) Brownian motion in biological membranes. Proc Natl Acad Sci USA 72(eight):3111113. 37. Chung I, et al. (2010) Spatial handle of EGF receptor activation by NF-κB Activator MedChemExpress reversible dimerization on living cells. nature 464(7289):78387. 38. Knight JD, Lerner MG, Marcano-Vel quez JG, Pastor RW, Falke JJ (2010) Single molecule diffusion of membrane-bound proteins: Window into lipid contacts and bilayer dynamics. Biophys J 99(9):2879887. 39. Kaizuka Y, Groves JT (2004) Structure and dynamics of supported intermembrane junctions. Biophys J 86(two):90512. 40. Goodwin JS, Drake KR, Remmert CL, Kenworthy AK (2005) Ras diffusion is sensitive to plasma membrane viscosity. Biophys J 89(2):1398410. 41. Hall BE, Yang SS, Boriack-Sjodin PA, Kuriyan J, Bar-Sagi D (2001) Structure-based mutagenesis reveals distinct functions for Ras switch 1 and switch two in Sos-catalyzed guanine nucleotide exchange. J Biol Chem 276(29):276297637. 42. Moertelmaier M, Brameshuber M, Linimeier M, Schutz GJ, Stockinger H (2005) Thinning out clusters though conserving stoichiometry of labeling. Appl Phys Lett 87(26):263903. 43. Suhling K, Davis DM, Phillips D (2002) The influence of solvent viscosity on the fluorescence decay and time-resolved anisotropy of green fluorescent protein. J Fluoresc 12(1):915. 44. Hink MA, et al. (2000) Structural dynamics of green fluorescent protein alone and fused having a single chain Fv protein. J Biol Chem 275(23):175567560. 45. Spille J-H, Z n A, Hoffmann C, Lohse MJ, Harms GS (2011) Rotational diffusion on the (2a) adrenergic receptor revealed by FlAsH labeling in living cells. Biophys J 100(four): 1139148. 46. Alexiev U, Rimke I, P lmann T (2003) Elucidation of the nature of the conformational changes from the EF-interhelical loop in bacteriorhodopsin and of your helix VIII around the cytoplasmic surface of bovine rhodopsin: A time-resolved fluorescence depolarization study. J Mol Biol 328(three):70519. 47. Naji A, mTORC1 Activator Formulation Levine AJ, Pincus PA (2007) Corrections towards the Saffman-Delbruck mobility for membrane bound proteins. Biophys J 93(11):L49 51. 48. M ler JD, Chen Y, Gratton E (2000) Resolving heterogeneity o.
