Name :
Anti-BACE1 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
Rabbit polyclonal to BACE1
Tested applications :
ELISA, WB, IHC-P
Species reactivity :
Mouse BACE1
Alternative names :
ASP2 antibody; BACE antibody; HSPC104 antibody; C76936 antibody; ASP2 antibody; BACE antibody; BACE1 antibody; Bace1 antibody; C76936 antibody; FLJ90568 antibody; HSPC104 antibody; KIAA1149 antibody
Immunogen :
Mouse BACE1 (His Tag) recombinant protein
Isotype :
Rabbit IgG
Preparation :
Produced in rabbits immunized with purified, recombinant Mouse BACE1 (rM BACE1; NM_011792.5; Met1-Thr457). BACE1 specific IgG was purified by Mouse BACE1 affinity chromatography.
Clonality :
Polyclonal
Formulation :
0.2 μm filtered solution in PBS
Storage instructions :
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Applications :
WB: 1-5 μg/mlELISA: 0.1-0.2 μg/mlThis antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Mouse BACE1.IHC-P: 0.1-2 μg/mL
Background :
Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain, This protein is expressed highly in the substantia nigra, locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer’s disease. In Alzheimer’s disease patients, BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca(2+) (microM range) significantly increased the proteolytic activity of BACE1. Furthermore, BACE1 protein is ubiquitinated, and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production.
References :
Christensen MA, et al. (2004) Transcriptional regulation of BACE1, the beta-amyloid precursor protein beta-secretase, by Sp1. Mol Cell Biol. 24(2):865-74. Stockley JH, et al. (2007) The proteins BACE1 and BACE2 and beta-secretase activity in normal and Alzheimer’s disease brain. Biochem Soc Trans. 35(Pt 3): 574-6. Savonenko AV, et al. (2008) Alteration of BACE1-dependent NRG1/ErbB4 signaling and schizophrenia-like phenotypes in BACE1-null mice. Proc Natl Acad Sci U S A. 105(14): 5585-90. Hayley M, et al. (2009) Calcium enhances the proteolytic activity of BACE1: An in vitro biophysical and biochemical characterization of the BACE1-calcium interaction. Biochim Biophys Acta. 1788(9): 1933-8.
Related websites: https://www.medchemexpress.com/antibodies.html
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