Name :
Anti-BCL2 / Bcl-2 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
Mouse monoclonal to BCL2 / Bcl-2
Tested applications :
WB, IP
Species reactivity :
Human BCL2 / Bcl-2
Alternative names :
Bcl-2 antibody; PPP1R50 antibody; Bcl-2 antibody; AW986256 antibody; C430015F12Rik antibody; D630044D05Rik antibody; D830018M01Rik antibody; apoptosis regulator Bcl-2 antibody; apoptosis regulator Bcl-2 antibody; AW986256 antibody; B-cell leukemia/lymphoma 2 antibody; Bcl-2 antibody; Bcl-2 antibody; C430015F12Rik antibody; D630044D05Rik antibody; D830018M01Rik antibody; PPP1R50 antibody; protein phosphatase 1 antibody; regulatory subunit 50 antibody
Immunogen :
Human BCL2 / Bcl-2 (His Tag) recombinant protein
Isotype :
Mouse IgG1
Preparation :
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BCL2 / Bcl-2 (rh BCL2 / Bcl-2; P10415; Met1-Asp211). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Clonality :
Monoclonal
Formulation :
0.2 μm filtered solution in PBS with 5% trehalose
Storage instructions :
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Applications :
WB: 3-5 μg/mLIP: 2-8 μg/mg of lysate
Background :
BCL2 (B-cell leukemia/lymphoma 2, N-Histidine-tagged), also known as Bcl-2, belongs to the Bcl-2 family. Bcl-2 family proteins regulate and contribute to programmed cell death or apoptosis. It is a large protein family and all members contain at least one of four BH (bcl-2 homology) domains. Certain members such as Bcl-2, Bcl-xl and Mcl1 are anti-apoptotic, whilst others are pro-apoptotic. Most Bcl-2 family members contain a C-terminal transmembrane domain that functions to target these proteins to the outer mitochondrial and other intracellular membranes. It is expressed in a variety of tissues. BCL2 blocks the apoptotic death of some cells such as lymphocytes. It also regulates cell death by controlling the mitochondrial membrane permeability and inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
References :
Tsujimoto Y, et al. (1984) Cloning of the chromosome breakpoint of neoplastic B cells with the t(14;18) chromosome translocation. Science. 226(4678):1097-99. Cleary ML, et al. (1986) Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation. Cell. 47(1):19-28. Otake Y, et al. (2007) Overexpression of nucleolin in chronic lymphocytic leukemia cells induces stabilization of Bcl-2 / Bcl-2 mRNA. Blood. 109(7):3069-75.
Related websites: https://www.medchemexpress.com/antibodies.html
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