Name :
Hemoglobin alpha antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
Hemoglobin alpha Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in K-562 cells, mouse liver tissue, rat brain tissue, rat heart tissue. Observed molecular weight by Western-blot: 14-16 kDa, 27 kDa
Tested applications :
ELISA, WB, IP
Species reactivity :
Human, Mouse, Rat; other species not tested.
Alternative names :
Alpha globin antibody; HBA1 antibody; HBA2 antibody; Hemoglobin a antibody; Hemoglobin alpha chain antibody; Hemoglobin subunit alpha antibody; hemoglobin antibody; alpha 1 antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of Hemoglobin alpha recombinant protein (Accession Number: NM_000558). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:200-1:2000
Background :
The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (20836851). This antibody may cross-react with other hemoglobin chains.
References :
Yang G, Shi W, Hu X. Therapeutic effects of induced pluripotent stem cells in chimeric mice with β-thalassemia. Haematologica. 99(8):1304-11. 2014. Homma T, Kurahashi T, Lee J, Kang ES, Fujii J. SOD1 deficiency decreases proteasomal function, leading to the accumulation of ubiquitinated proteins in erythrocytes. Archives of biochemistry and biophysics. 583:65-72. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
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