Name :
HuR antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
HuR Rabbit Polyclonal antibody. Positive FC detected in MCF-7 cells. Positive IHC detected in human ovary tumor tissue, human breast cancer tissue, human pancreas tissue, human skin tissue. Positive IF detected in MCF-7 cells, Ethacrynic acid treated HepG2 cells. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, A431 cells, HeLa cells, hESC cells, HL-60 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, RAW264.7 cells. Observed molecular weight by Western-blot: 30-37kd
Tested applications :
ELISA, WB, IF, IHC, FC, IP
Species reactivity :
Human,Mouse,Rat,Zebrafish; other species not tested.
Alternative names :
ELAV like protein 1 antibody; ELAV1 antibody; ELAVL1 antibody; Hu antigen R antibody; Hua antibody; HuR antibody; MelG antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of HuR recombinant protein (Accession Number: NM_001419). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:500-1:5000IHC: 1:20-1:200IF: 1:20-1:200
Background :
ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3′-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
References :
Luo NA, Qu YQ, Yang GD. Post-transcriptional up-regulation of PDGF-C by HuR in advanced and stressed breast cancer. International journal of molecular sciences. 15(11):20306-20. 2014. Wu S, Wang Y, Lin L. Protease 2A induces stress granule formation during coxsackievirus B3 and enterovirus 71 infections. Virology journal. 11:192. 2014. Wang Y, Yue D, Xiao M. C1QBP negatively regulates the activation of oncoprotein YBX1 in the renal cell carcinoma as revealed by interactomics analysis. Journal of proteome research. 14(2):804-13. 2015. Li H, Hou S, Hao T. HuR antagonizes the effect of an intronic pyrimidine-rich sequence in regulating WT1 +/-KTS isoforms. RNA biology. 12(12):1364-71. 2015. Kuś K, Rakus K, Boutier M. The Structure of the Cyprinid herpesvirus 3 ORF112-Zα·Z-DNA Complex Reveals a Mechanism of Nucleic Acids Recognition Conserved with E3L, a Poxvirus Inhibitor of Interferon Response. The Journal of biological chemistry. 290(52):30713-25. 2015. Zhou J, Yang L, Zhong T. H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase. Nature communications. 6:10221. 2015. Zhao W, Zhao J, Hou M. HuR and TIA1/TIAL1 are involved in regulation of alternative splicing of SIRT1 pre-mRNA. International journal of molecular sciences. 15(2):2946-58. 2014. Wu S, Lin L, Zhao W. AUF1 is recruited to the stress granules induced by coxsackievirus B3. Virus research. 192:52-61. 2014.
Related websites: https://www.medchemexpress.com/antibodies.html
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