Name :
Anti-BLMH/Bleomycin Hydrolase antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
Rabbit polyclonal to BLMH/Bleomycin Hydrolase
Tested applications :
ELISA, WB, IP
Species reactivity :
Mouse BLMH / Bleomycin Hydrolase
Alternative names :
AI035728 antibody; BH antibody; Bleomycin Hydrolase antibody; Blmh antibody; BMH antibody; RP23-100P23.3 antibody; Bh antibody; Bleomycin Hydrolase antibody; BLMH antibody; Bmh antibody; MGC36899 antibody; MGC37104 antibody; MGC39026 antibody; BH antibody; BMH antibody; Bh antibody; Bmh antibody; AI035728 antibody
Immunogen :
Mouse BLMH/Bleomycin Hydrolase (His Tag) recombinant protein
Isotype :
Rabbit IgG
Preparation :
Produced in rabbits immunized with purified, recombinant Mouse BLMH / Bleomycin Hydrolase (rM BLMH / Bleomycin Hydrolase; NP_848760.1; Asn 2-Glu 455). BLMH / Bleomycin Hydrolase specific IgG was purified by Mouse BLMH / Bleomycin Hydrolase affinity chromatography.
Clonality :
Polyclonal
Formulation :
0.2 μm filtered solution in PBS with 5% trehalose
Storage instructions :
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Applications :
WB: 10-20 μg/mLELISA: 0.1-0.2 μg/mLThis antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse BLMH. The detection limit for Mouse BLMH is approximately 0.00245 ng/well.IP: 1-4 μg/mg of lysate
Background :
The papain superfamily member bleomycin hydrolase (BLMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution. The only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The papain superfamily member bleomycin hydrolase (BLMH) is a neutral cysteine protease with structural similarity to a 20S proteasome. Bleomycin (BLM), a clinically used glycopeptide anticancer agent. BLMH is an essential protectant against BLM-induced death and has an important role in neonatal survival and in maintaining epidermal integrity. Sequencing revealed several putative sites phosphorylated by different types of protein kinases, but no signal sequence, transmembrane domain, N-linked glycosylation site or DNA-binding motif.
References :
Takeda A, et al. (1996) Cloning and Analysis of cDNA Encoding Rat Bleomycin Hydrolase, a DNA-Binding Cysteine Protease. J Biochem. 120 (2): 353-9. Lefterov IM, et al. (2000) Human bleomycin hydrolase regulates the secretion of amyloid precursor protein. The FASEB Journal. 14(12): 1837-47. Brmme D, et al. (1996) Human Bleomycin Hydrolase: Molecular Cloning, Sequencing, Functional Expression, and Enzymatic Characterization. Biochemistry. 35 (21): 6706-14.
Related websites: https://www.medchemexpress.com/antibodies.html
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