Name :
IFT25 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
IFT25 Rabbit Polyclonal antibody. Positive IF detected in hTERT-RPE1 cells. Positive WB detected in HeLa cells, A549 cells, mouse brain tissue. Observed molecular weight by Western-blot: 25 kDa
Tested applications :
ELISA, WB, IF
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
C1orf41 antibody; Heat shOCk protein beta 11 antibody; HSPB11 antibody; HSPCO34 antibody; IFT25 antibody; ITF25 antibody; Placental protein 25 antibody; PP25 antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of IFT25 recombinant protein (Accession Number: NM_016126). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IF: 1:20-1:200
Background :
HSPB11 (Heat shock protein beta-11), also known as IFT25, was originally identified as a small heat shock protein. Recently it has been identified as a component of IFT complex B. It has been demonstrated that IFT25 is not required for ciliary assembly but is required for proper Hedgehog signaling, which in mammals occurs within cilia. Cilia lacking IFT25 have defects in the signal-dependent transport of multiple Hedgehog components and fail to activate the pathway upon stimulation.
References :
Raman M, Sergeev M, Garnaas M. Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis. Nature cell biology. 17(10):1356-69. 2015. Cheng W, Li M, Jiang Y. Association between small heat shock protein B11 and the prognostic value of MGMT promoter methylation in patients with high-grade glioma. Journal of neurosurgery. 2015. Keady BT, Samtani R, Tobita K. IFT25 links the signal-dependent movement of Hedgehog components to intraflagellar transport. Developmental cell. 22(5):940-51. 2012. Eguether T, San Agustin JT, Keady BT. IFT27 links the BBSome to IFT for maintenance of the ciliary signaling compartment. Developmental cell. 31(3):279-90. 2014. Yang N, Li L, Eguether T, Sundberg JP, Pazour GJ, Chen J. Intraflagellar transport 27 is essential for hedgehog signaling but dispensable for ciliogenesis during hair follicle morphogenesis. Development (Cambridge, England). 142(12):2194-202. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
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