Name :
IFT52 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
IFT52 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive WB detected in MDCK cells, human testis tissue, mouse testis tissue. Positive IP detected in mouse testis tissue, HeLa cells. Observed molecular weight by Western-blot: 50kd
Tested applications :
ELISA, IF, WB, IP
Species reactivity :
Human,Mouse,Rat, Dog; other species not tested.
Alternative names :
C20orf9 antibody; CGI 53 antibody; dJ1028D15.1 antibody; IFT52 antibody; NGD5 antibody; Protein NGD5 homolog antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of IFT52 recombinant protein (Accession Number: NM_016004). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:200-1:2000IP: 1:500-1:5000IF: 1:10-1:100
Background :
IFT52, also named as C20orf9 and NGD5, is a component of IFT complex B which is composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. IFT52 binds directly to the IFT81/74/27/25 complex. And IFT52 could mediate the interaction between the two subcomplexes.(PMID:21642430) The MW of IFT52 is about 50-54kd.
References :
Delaval B, Bright A, Lawson ND, Doxsey S. The cilia protein IFT88 is required for spindle orientation in mitosis. Nature cell biology. 13(4):461-8. 2011. Cevik S, Sanders AA, Van Wijk E. Active transport and diffusion barriers restrict Joubert Syndrome-associated ARL13B/ARL-13 to an Inv-like ciliary membrane subdomain. PLoS genetics. 9(12):e1003977. 2013. Xu Y, Cao J, Huang S. Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function. PloS one. 10(4):e0124378. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
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