Name :
IkB alpha antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
IkB alpha Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue, human prostate cancer tissue. Positive IF detected in Hela cells. Positive FC detected in HeLa cells, NIH/3T3 cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, A431 cells, HEK-293 cells, HL-60 cells, Jurkat cells, mouse liver tissue, NIH/3T3 cells, pig liver tissue. Observed molecular weight by Western-blot: 36-40 kDa
Tested applications :
ELISA, IHC, IF, WB, FC, IP
Species reactivity :
Human,Mouse,Rat, Pig; other species not tested.
Alternative names :
I kappa B alpha antibody; IkappaBalpha antibody; IkB alpha antibody; IKBA antibody; MAD 3 antibody; MAD3 antibody; NF kappa B inhibitor alpha antibody; NFKBI antibody; NFKBIA antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of IkB alpha recombinant protein (Accession Number: NM_020529). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:200-1:2000IP: 1:500-1:5000IHC: 1:20-1:200IF: 1:10-1:100
Background :
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
References :
Tang S, Bao H, Zhang Y, Yao J, Yang P, Chen X. 14-3-3ε mediates the cell fate decision-making pathways in response of hepatocellular carcinoma to Bleomycin-induced DNA damage. PloS one. 8(3):e55268. 2013. Zhang L, Tang H, Kou Y. MG132-mediated inhibition of the ubiquitin-proteasome pathway ameliorates cancer cachexia. Journal of cancer research and clinical oncology. 139(7):1105-15. 2013. Luo J, Zhou H, Wang F. The hepatitis B virus X protein downregulates NF-κB signaling pathways through decreasing the Notch signaling pathway in HBx-transformed L02 cells. International journal of oncology. 42(5):1636-43. 2013. Li Y, Shen D, Tang X. Chlorogenic acid prevents isoproterenol-induced hypertrophy in neonatal rat myocytes. Toxicology letters. 226(3):257-63. 2014. Wang K, Ni L, Wang S, Zheng C. Herpes simplex virus 1 protein kinase US3 hyperphosphorylates p65/RelA and dampens NF-κB activation. Journal of virology. 88(14):7941-51. 2014. Shi X, Chen G, Liu X. Scutellarein inhibits cancer cell metastasis in vitro and attenuates the development of fibrosarcoma in vivo. International journal of molecular medicine. 35(1):31-8. 2015. Wang F, Yang JL, Yu KK. Activation of the NF-κB pathway as a mechanism of alcohol enhanced progression and metastasis of human hepatocellular carcinoma. Molecular cancer. 14:10. 2015. Liu M, Xu L, Yin L. Potent effects of dioscin against obesity in mice. Scientific reports. 5:7973. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
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