Name :
ISCU antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
ISCU Rabbit Polyclonal antibody. Positive IP detected in mouse heart tissue. Positive WB detected in mouse heart tissue, COLO 320 cells, HeLa cells. Positive IHC detected in human lung cancer tissue, human endometrial cancer tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 14-15 kDa,18-19 kDa
Tested applications :
ELISA, WB, IHC, IF, IP
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
2310020H20Rik antibody; HML antibody; hnifU antibody; ISCU antibody; ISU2 antibody; NIFU antibody; NifU like protein antibody; NIFUN antibody
Immunogen :
Human ISCU Recombinant protein (GST tag)
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of ISCU recombinant protein (Accession Number: NM_213595). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:200-1:2000IP: 1:200-1:2000IHC: 1:20-1:200IF: 1:20-1:200
Background :
Iron-sulfur (Fe-S) clusters are required for the functions of mitochondrial aconitase, mammalian iron regulatory protein 1, and many other proteins in multiple subcellular compartments. Mitochondrial and cytosolic isoforms of the human Fe-S cluster scaffold protein, iron-sulfur cluster assembly enzyme (ISCU), is involved in activation of iron uptake, redistribution of intracellular iron, and decreased utilization of iron in Fe-S proteins. Defects in ISCU gene are the cause of myopathy with exercise intolerance Swedish type (MEIS), which is characterized by lifelong severe exercise intolerance (PMID: 18304497). Two ISCU isoforms ISCU1 (cytosolic, 15 kDa) and ISCU2 (mitochondrial, 18 kDa), were produced by alternative splicing, and ISCU2 is considered the canonical isoform1. ISCU2 protein is rapidly processed upon maturation (PMID: 11060020). Proteintech’s ISCU antibody 14812-1-AP is raised against the full-length ISCU2 and is expected to detect 14 kDa,15 kDa and 18-19 kDa bands which has been reported in the previous observation (PMID: 11060020).
References :
Ferecatu I, Gonçalves S, Golinelli-Cohen MP. The diabetes drug target MitoNEET governs a novel trafficking pathway to rebuild an Fe-S cluster into cytosolic aconitase/iron regulatory protein 1. The Journal of biological chemistry. 289(41):28070-86. 2014. Stehling O, Vashisht AA, Mascarenhas J. MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity. Science (New York, N.Y.). 337(6091):195-9. 2012. Funauchi Y, Tanikawa C, Yi Lo PH. Regulation of iron homeostasis by the p53-ISCU pathway. Scientific reports. 5:16497. 2015. Maio N, Singh A, Uhrigshardt H, Saxena N, Tong WH, Rouault TA. Cochaperone binding to LYR motifs confers specificity of iron sulfur cluster delivery. Cell metabolism. 19(3):445-57. 2014. Qiao A, Khechaduri A, Kannan Mutharasan R, Wu R, Nagpal V, Ardehali H. MicroRNA-210 decreases heme levels by targeting ferrochelatase in cardiomyocytes. Journal of the American Heart Association. 2(2):e000121. 2013.
Related websites: https://www.medchemexpress.com/antibodies.html
Popular product recommendations:
Hexokinase II Antibody
BAX Antibody (YA825)
eIF4EBP1 Antibody: eIF4EBP1 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 13 kDa, targeting to eIF4EBP1. It can be used for WB,ICC/IF,IHC-P,IP,FC assays with tag free, in the background of Human, Mouse, Rat.
