Name :
ISG15 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
ISG15 Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer tissue, human pancreas cancer tissue. Positive IP detected in HeLa cells. Positive WB detected in human brain tissue. Observed molecular weight by Western-blot: 15-17 kDa
Tested applications :
ELISA, WB, IHC, IP
Species reactivity :
Human; other species not tested.
Alternative names :
G1P2 antibody; hUCRP antibody; IFI15 antibody; IP17 antibody; ISG15 antibody; ISG15 ubiquitin like modifier antibody; Ubiquitin like protein ISG15 antibody; UCRP antibody
Immunogen :
Human ISG15 Recombinant protein (GST tag)
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of ISG15 recombinant protein (Accession Number: NM_005101). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:200-1:2000IP: 1:200-1:2000IHC: 1:20-1:200
Background :
ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA) and -beta (IFNB). ISG15 forms covalent conjugates with its target proteins in a process called ISGylation, which in mammals is known to play a role in antiviral immunity. ISG15 proteins possess two ubiquitin-like (UBL) domains and a highly conserved C-terminal LRGG sequence, the latter being known as the ubiquitin conjugation motif. Intracellular ISG15 are conjugated, via the LRGG motif, to target proteins through a process called ISGylation, which resembles largely ubiquitination, the process of formation of ubiquitin conjugates. Unconjugated extracellular ISG15, which are released from several types of human and murine cells, are known to possess cytokine-like activity.
References :
Zhang W, Zhang L, Wu Z, Tien P. Differential interferon pathway gene expression patterns in Rhabdomyosarcoma cells during Enterovirus 71 or Coxsackievirus A16 infection. Biochemical and biophysical research communications. 447(3):550-5. 2014. Sainz B, Martín B, Tatari M, Heeschen C, Guerra S. ISG15 is a critical microenvironmental factor for pancreatic cancer stem cells. Cancer research. 74(24):7309-20. 2014.
Related websites: https://www.medchemexpress.com/antibodies.html
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